We are here to help guide your Microbiome research by providing expertise and technical assistance with sample processing, library prep and analysis. Sequencing will be provided by the Duke Sequencing and Genomic Technologies (SGT) core facility. We also provide access to the NanoString nCounter system to support your gene expression experiments and the GeoMx Digital Spatial Profiler for spatial transcriptomics.
Step 1: Schedule a consultation with us by emailing us at microbiomesr@duke.edu prior to your experiment and sample submission. We provide experimental design, sample collection and procedure protocols, pre- and post-grant support and manuscript support.
Step 2: Review papers to guide experimental design of your microbiome project:
- Guidelines regarding human subjects protection and microbiome research
- Best practices for analyzing microbiomes
- Conducting a microbiome study
- Controls to consider for Microbiome Research
- Microbiome 101: Studying, analyzing and interpreting gut microbiome data for clinicians
- 16S data analysis approach using QIIME 2
- Time of sample collection is critical for the replicability of microbiome analyses
Step 3: Obtain quote for services needed for your research.
Step 4: Submit service request and sample information via our online submission form.
Step 5: Make an appointment to submit samples at MSRB3 by emailing microbiomesr@duke.edu.
Sample Drop Off guidelines
- Samples must be dropped off at a pre-arranged time. Please make sure to set this up before bringing samples to MSRB3.
- All samples must be submitted with a copy of the order form.
- Email microbiomesr@duke.edu or call one of the staff to notify us when you arrive at MSRB3.
- A staff member from the Microbiome Core Facility will meet you near the entrance with a cooler.