Microbiome Core Facility Sample Requirements

Sample collection and storage

• Samples should be collected, transported and stored in format that preserves microbiome integrity
  • e.g. Omnigene or Norgen tubes, stool/swabs/filters at -80 C
  • include multiple collection negative controls with low biomass samples
  • aliquoting is performed in hood to minimize background contamination
• human samples must be de-identified before submission 
• please contact us if you have questions on best practices for sample collection and storage

Nucleic Acid extraction

• Standard DNA extractions will be done using Qiagen Powersoil Pro kit on the Qiacube HT
  • extractions using alternate kits available, please contact us for details
• Provide at least 0.1g of tissue in eppendorf tube stored at -80C
• Provide at least 250ul of liquid (blood, water or body fluid) stored in -80C
• Pelleted cells in 1.5ml eppendorf tube stored in -80C

Amplicon Library Prep for NGS

Extracted DNA NGS library prep for amplicon library prep can be submitted in eppendorf tubes or 96 well plates.

All sequencing is performed by the Duke Sequencing and Genomic Technologies Core. Sequencing requests and cost estimates can be made through SeqLims. For amplicon libraries and DNA extractions done by the Microbiome core, we will prepare sequencing submissions for you.

• Provide 4 uls of sample in eppendorf tubes that are clearly labeled
• DNA must be stored at -20C
• RNA must be stored at -80C
• if necessary, please dilute sample to appropriate concentration range