Brook Heaton

Heaton – Lab Members

Headshot of Brook Heaton_Heaton LabResearch Assistant Professor
218 CARL Building
Box 3054
Durham, NC 27710
Phone: (919) 684-1375
Fax: (919) 684-2790
brook.heaton@dm.duke.edu

Research Interest:

I received my PhD from the University of Chicago where I studied toxin/antitoxin systems in Brucella abortus. I then did my post-doctoral training at Memorial Sloan-Kettering and studied DNA repair in Mycobacterium tuberculosis.   I have now jumped ship to become a virologist and my project focuses on understanding the mechanisms by which influenza viruses cause disease.

Personal Interests:

My interests outside of lab include knitting and finding new bakeries and restaurants to try.

Publications List:

YaronTM*, Heaton BE*§, Levy TM*, Johnson JL*,  Jordan TX*, Cohen BM, Kerelsky A, Lin T, Liberatore KM, Bulaon DK, Kastenhuber ER, Mercadante MN, Shobana-Ganesh K, He L, Schwartz RE, Chen S, Weinstein H, Elemento O, Piskounova E, Nilsson-Payant BE, Lee G, Trimarco JD, Burke KN, Hamele CE, Chaparian RR, Harding AT, Tata A, Zhu X, Tata PR, Smith CM, Possemato AP, Tkachev SL, Hornbeck PV, Beausoleil SA, Anand SK, Aguet F, Getz G, Davidson AD, Heesom K, Kavanagh-Williamson M, Matthews D, tenOever BR§, Cantley LC§, Blenis J§, Heaton NS§.  The FDA-approved drug Alectinib compromises SARS-CoV-2 nucleocapsid phosphorylation and inhibits viral infection in vitro.  bioRxiv 2020.08.14.251207; doi: https://doi.org/10.1101/2020.08.14.251207
* Authors contributed equally
§Corresponding authors

Katsura H, Sontake V, Tata A, Kobayashi Y, Edwards CE, Heaton BE, Konkimalla A, Asakura T, Mikami Y, Fritch EJ, Lee PJ, Heaton NS, Boucher RC, Randell SH, Baric RS, Tata PR. Human Lung Stem Cell-Based Alveolospheres Provide Insights into SARS-CoV-2-Mediated Interferon Responses and Pneumocyte Dysfunction. Cell Stem Cell. 2020 Oct 21:S1934-5909(20)30499-9. doi: 10.1016/j.stem.2020.10.005. Epub ahead of print. PMID: 33128895.

Froggatt HM, Heaton BE, Heaton NS. Development of a fluorescence based, high-throughput SARS-CoV-2 3CLpro reporter assay. J Virol. 2020 Aug 25:JVI.01265-20. doi: 10.1128/JVI.01265-20.  PMID: 32843534

Chambers BS, Heaton BE, Rausch K, Dumm RE, Hamilton JR, Cherry S, Heaton NS.  DNA mismatch repair is required for the host innate response and controls cellular fate after influenza virus infection.  2019.  Nature Microbiology.  4(11):1964-1977.  PMID: 31358986

Wipperman MF*, Heaton BE*, Nautiyal A*, Adefisayo O*, Evans H, Gupta R, van Ditmarsch D, Soni R, Hendrickson R, Johnson J, Krogan N, Glickman MS. Mycobacterial Mutagenesis and Drug Resistance Are Controlled by Phosphorylation- and Cardiolipin- Mediated Inhibition of the RecA Coprotease. Molecular Cell. 2018 *Authors contributed equally

Heaton BE, Kennedy EM, Dumm RE, Harding AT, Sacco MT, Sachs D, Heaton NS. A CRISPR Activation Screen Identifies a Pan-avian Influenza Virus Inhibitory Host Factor. Cell Reports. 2017 20(7): p1503-1512. http://dx.doi.org/10.1016/j.celrep.2017.07.060 PMID: 28813663

Harding AT, Heaton BE, Dumm RE, Heaton NS. Rationally Designed Influenza Virus Vaccines That Are Antigenically Stable during Growth in Eggs. mBio. 2017 June 6, Vol. 8 no.3 e00669-17 doi: 10.1128/mBio.00669-17 PMID: 28588131

Heaton BE, Barkan D, Bongiorno P, Karakousis PC, Glickman MS. “Deficiency of double-strand DNA break repair does not impair Mycobacterium tuberculosis virulence in multiple animal models of infection” 2014. Infection and Immunity. 82(8):3177-85. PMCID: PMC4136208

Heaton BE, Herrou J, Blackwell A, Wysocki V, and Crosson S. “Molecular structure and function of the novel BrnT/BrnA toxin-antitoxin system of Brucella abortus” 2012. Journal of Biological Chemistry. 287:12098-12110. PMCID: PMC3320955

Ragle BE, Karginov VA, and Bubeck Wardenburg J. “Prevention and treatment of Staphylococcus aureus pneumonia with a beta-cyclodextrin derivative. 2010. Antimicrobial Agents and Chemotherapy. 54(1):298-304. PMCID: PMC2798498

Ragle BE, Bubeck Wardenburg J. “Anti-α-hemolysin monoclonal antibodies mediate protection against Staphylococcus aureus pneumonia.” 2009. Infection and Immunity. 77(7):2712-8. PMCID: PMC2708543

Dos Santos PC, Johnson DC, Ragle BE, Unciuleac MC, Dean DR. “Controlled expression of nif and isc iron-sulfur protein maturation components reveals target specificity and limited functional replacement between the two systems.” 2007.  J Bacteriol.  189(7):2854-62. PMCID: PMC1855825