Tim Smith

MGM Graduate Student (2018)Tim Smith
Asokan Lab
5200 MSRBIII, 2653 DUMC
Phone: 919-613-6693
Fax: 919-684-2790



Research Interests: Molecular Virology | Adeno-Associated Virus | Gene Therapy

In the Asokan Lab we are interested in building innovative technology platforms for gene therapy and gene editing.  One aspect of my research relies heavily on the use of advanced molecular and cell biology techniques to probe fundamental aspects of adeno-associated virus biology, particularly virus-receptor interaction. The other aspect explores structural biology and directed evolution to engineer novel viral vectors to treat genetic disease.

Personal: New York native. Concert junkie. Passionate ski trip planner. Ocean enthusiast. Occasional hockey player. When not in lab, I like hiking, camping, and exploring Durham’s public houses with new friends here at Duke.


Chen PH, Hu J, Wu J, Huynh DT, Smith TJ, Pan S, Bisnett BJ, Smith AB, Lu A, Condon BM, Chi JT and Boyce M. Gigaxonin glycosylation regulates intermediate filament turnover and may impact giant axonal neuropathy etiology or treatment. JCI insight. 2020;5(1).

Chen PH, Smith T, Hu J, Pan S, Smith A, Lu A, JT Chi, M Boyce. Glycosylation of gigaxonin regulates intermediate filaments: Novel molecular insights into giant axonal neuropathy. bioRxiv. 2019;530303.

Toleman CA*, Schumacher MA*, Yu SH, Zeng W, Cox NJ, Smith TJ, Soderblom EJ, Wands AM, Kohler JJ and Boyce M. Structural basis of O-GlcNAc recognition by mammalian 14-3-3 proteins. Proceedings of the National Academy of Sciences. 2018;115(23):5956–61.

Tarbet HJ, Dolat L, Smith TJ, Condon BM, O’Brien ET, Valdivia RH and Boyce M. Site-specific glycosylation regulates the form and function of the intermediate filament cytoskeleton. Elife. 2018;7:e31807.

Cox NJ, Unlu G, Bisnett BJ, Meister TR, Condon BA, Luo PM, Smith TJ, Hanna M, Chhetri A, Soderblom EJ, Audhya A, Knapik EW and Boyce M. Dynamic glycosylation governs the vertebrate COPII protein trafficking pathway. Biochemistry. 2018;57(1):91–107.

Cox NJ, Luo PM, Smith TJ, Bisnett BJ, Soderblom EJ, Boyce M. A Novel Glycoproteomics Workflow Reveals Dynamic O-GlcNAcylation of COPγ1 as a Candidate Regulator of Protein Trafficking. Frontiers in endocrinology. 2018;9:606.

Chen PH, Smith TJ, Wu J, Siesser PF, Bisnett BJ, Khan F, Hogue M, Soderblom E, Tang F, Marks JR, Major MB, Swarts BM, Boyce M* and Chi JT. Glycosylation of KEAP1 links nutrient sensing to redox stress signaling. The EMBO journal. 2017;36(15):2233–50.

Bragg JT, D’Ambrosio HK, Smith TJ, Gorka CA, Khan FA, Rose JT, Rouff AJ, Fu TS, Bisnett BJ, Boyce M, Khetan S and Paulick MG. Esterified Trehalose Analogues Protect Mammalian Cells from Heat Shock. ChemBioChem. 2017;18(18):1863–70.

Palaniappan KK, Hangauer MJ, Smith TJ, Smart BP, Pitcher AA, Cheng EH, Bertozzi CR, Boyce M. A chemical glycoproteomics platform reveals O-GlcNAcylation of mitochondrial voltage-dependent anion channel 2. Cell reports. 2013;5(2):546–52.