John H. McCusker, PhD – Publications

Associate Professor

research  biography • lab members • publications

Google Scholar Profile 

Representative Publications:

Vijayraghavan S, Kozmin SG, Strope PK, Skelly DA, Lin Z, Kennell J, Magwene PM, Dietrich FS, McCusker JH.  Mitochondrial Genome Variation Affects Multiple Respiration and Non-respiration Phenotypes in Saccharomyces cerevisiae.  Genetics. 2018 Nov 29. pii: genetics.301546.2018. doi: 10.1534/genetics.118.301546. [Epub ahead of print]

Strope PK, Kozmin SG, Skelly DA, Magwene PM, Dietrich FS, McCusker JH. 2μ plasmid in Saccharomyces species and in Saccharomyces cerevisiae. FEMS Yeast Res. Dec 2015 15(8). pii: fov090. doi: 10.1093/femsyr/fov090. [Abstract]

Strope PK, Skelly DA, Kozmin SG, Mahadevan G, Stone EA, Magwene PM, Dietrich FS, McCusker JH.  The 100-genomes strains, an S. cerevisiae resource that illuminates its natural phenotypic and genotypic variation and emergence as an opportunistic pathogen. Genome Res. May 25:762-74. 2015 Apr 3. [PDF@Journal]

Zhao Y., P.K. Strope, S.G. Kozmin, J.H. McCusker, F.S. Dietrich, R.J. Kokoska, T.D. Petes. 2014. Structures of naturally-evolved CUP1 Tandem arrays in yeast indicate that these arrays are generated by unequal non-homologous recombination. G3. Sep 4: 2259-69. [PDF@Journal]

Muller L.A.H., J.E. Lucas, D.R. Georgianna, and J.H. McCusker. 2011. Genome-wide association analysis of clinical vs. nonclinical origin provides insights into Saccharomyces cerevisiae pathogenesis. Molecular Ecology. 20:4085-4097. [epub 31 Aug 2011] Abstract

Esberg  A.,  L.A.H. Muller and J.H. McCusker. 2011. Genomic structure of and genome-wide recombination in the Saccharomyces cerevisiae S288C progenitor isolate EM93. PLoS ONE. 6(9):e25211. Abstract

Argueso, J.L., M.F. Carazzolle, P.A. Mieczkowski, F.M. Duarte, O.V. Netto, S.K. Missawa, F. Galzerani, G.G. Costa, R.O. Vidal, M.F. Noronha, M. Dominska, M.G. Andrietta, S.R. Andrietta, A.F. Cunha, L.H. Gomes, F.C. Tavares, A.R. Alcarde, F.S. Dietrich, J.H. McCusker, T.D. Petes, G.A. Pereira. 2009. Genome structure of a Saccharomyces cerevisiae strain widely used in bioethanol production. Genome Res. 19:2258-2270. [Epub 2009 Oct 7]. Abstract

Muller L.A. and J.H. McCusker. 2009. Microsatellite analysis of genetic diversity among clinical and nonclinical Saccharomyces cerevisiae isolates suggests heterozygote advantage in clinical environments. Mol Ecol. 18:2779-2786. [Epub 2009 May 20]. Abstract

Muller, L.A. and J.H. McCusker. 2009. A multispecies-based taxonomic microarray reveals interspecies hybridization and introgression in Saccharomyces cerevisiaeFEMS Yeast Res. 9:143-152. [Epub 2008 Nov 24]. Abstract

Sinha, H., L. David, R. C. Pascon, S. Clauder-Munster, S. Krishnakumar, M. Nguyen, G. Shi, J. Dean, R. W. Davis, P. J. Oefner, J. H. McCusker, and L. M. Steinmetz. 2008. Sequential elimination of major-effect contributors identifies additional quantitative trait loci conditioning high-temperature growth in yeast. Genetics180(3):1661-1670. [Epub 2008 Sep 9]. Abstract

Sinha, H., B.P. Nicholson, L.S. Steinmetz, and J.H. McCusker. 2006. Complex genetic interactions in a quantitative trait locus. PLoS Genetics 2(2):e13. Abstract

Steinmetz, L.M., H. Sinha, D.R. Richards, J.I. Spiegelman, P.J. Oefner, J.H. McCusker, and R.W. Davis. 2002. Dissecting the complex architecture of a quantitative trait locus in a yeast model. Nature 416:326-330. Abstract

Muller L.A.H. and J.H. McCusker. 2011. Nature and distribution of large sequence polymorphisms inSaccharomyces cerevisiaeFEMS Yeast Research. 11:587-594. [epub 8 Sep 2011] Abstract

Kingsbury, J. M., and J. H. McCusker. 2008. Threonine biosynthetic genes are essential in Cryptococcus neoformansMicrobiology 154:2767-2775. Abstract

Wei, W., J.H. McCusker, R.W. Hyman, T. Jones, Y. Ning, Z. Cao, Z. Gu, D. Bruno, M. Miranda, M. Nguyen, J. Wilhelmy, C. Komp, R. Tamse, X. Wang, P. Jia, P. Luedi, P.J. Oefner, L. David, F.S. Dietrich, Y. Li, R.W. Davis, and L.M. Steinmetz. 2007. Genome sequencing and comparative analysis of Saccharomyces cerevisiae strain YJM789. Proc Natl Acad Sci USA 104(31):12825-12830. [Epub 2007 Jul 25]. Abstract

McCusker, J.H., Chapter 18: Saccharomyces cerevisiae: an emerging and opportunistic pathogenic fungus, in Molecular Principles of Fungal Pathogenesis, J. Heitman, et al., Editors. 2006, ASM Press: Washington, D.C. p. 245-259.

Kingsbury J.M. and J.H. McCusker. 2010. Fungal homoserine kinase (thr1Δ) mutants are attenuated in virulence and die rapidly upon threonine starvation and serum incubation ∇. Eucaryotic Cell. 9:729-737. Abstract

Hartzog, P.E., B.P. Nicholson, and J.H. McCusker, Cytosine deaminase MX cassettes as positive/negative selectable markers in Saccharomyces cerevisiae. Yeast, 2005. 22: p. 789-798.

  1. Vorachek-Warren, M.K. and J.H. McCusker, DsdA (D-serine deaminase): a new heterologous MX cassette for gene disruption and selection in Saccharomyces cerevisiae. Yeast, 2004. 21(2): p. 163-71.

Kingsbury J.M. and J.H. McCusker. 2010. Homoserine toxicity in Saccharomyces cerevisiae and Candida albicans homoserine kinase (thr1Δ) mutants ∇. Eucaryotic Cell. 9:717-728. Abstract

Kingsbury, J.M. and J.H. McCusker. 2010. Cytocidal amino acid starvation of Saccharomyces cerevisiae andCandida albicans acetolactate synthase (ilv2{Delta}) mutants is influenced by the carbon source and rapamycin. Microbiology. 156:929-939. [Epub 2009 Dec 17]. Abstract

Kingsbury, J.M., A.L. Goldstein, and J.H. McCusker. 2006. The role of nitrogen and carbon transport, regulation, and metabolism genes for Saccharomyces cerevisiae survival in vivoEukaryotic Cell 5:816-824.Abstract

Pascon, R.C., T.M. Ganous, J.M. Kingsbury, G.M. Cox, and J.H. McCusker. 2004. Cryptococcus neoformansmethionine synthase: expression analysis and requirement for virulence. Microbiology 150:3013-3023.Abstract

Malkova, A., et al., Gene conversion and crossing over along the 405-kb left arm of Saccharomyces cerevisiae chromosome VII. Genetics, 2004. 168(1): p. 49-63.

Kingsbury, J.M., Z. Yang, T.M. Ganous, G.M. Cox, and J.H. McCusker. 2004. A novel chimeric spermidine synthase-saccharopine dehydrogenase ( SPE3-LYS9 ) gene in the human pathogen Cryptococcus neoformans.Eukaryotic Cell 3(3):752-763. Abstract

Kingsbury, J.M., Z. Yang, T.M. Ganous, G.M. Cox, and J.H. McCusker. 2004. Cryptococcus neoformans Ilv2p confers resistance to sulfometuron methyl and is required for survival at 37°C and in vivoMicrobiology150(5):1547-1558. Abstract

Ito-Harashima, S., P. Hartzog, H. Sinha, and J.H. McCusker. 2002. The tRNA-Tyr gene family of Saccharomyces cerevisiae: Agents of phenotypic variation and position effects on mutation frequency. Genetics 161(4):1395-1410. Abstract

Yang, Z., R.C. Pascon, J.A. Alspaugh, G.M. Cox, and J.H. McCusker. 2002. Molecular and genetic analysis of theCryptococcus neoformans MET3 gene and a met3 mutant. Microbiology 148:2617–2625. Abstract

Ito-Harashima, S. and J.H. McCusker, Positive and negative selection LYS5MX gene replacement cassettes for use in Saccharomyces cerevisiae. Yeast, 2004. 21(1): p. 53-61.

  1. Yang, Z., et al., Molecular and genetic analysis of the Cryptococcus neoformans MET3 gene and a met3 mutant. Microbiology, 2002. 148(Pt 8): p. 2617-25.

Goldstein, A.L., and J.H. McCusker. 2001. Development of Saccharomyces cerevisiae as a model pathogen: A system for the genetic identification of gene products required for survival in the mammalian host environment. Genetics 159:499-513. Abstract

Cruz, M.C., et al., Rapamycin and less immunosuppressive analogs are toxic to Candida albicans and Cryptococcus neoformans via FKBP12-dependent inhibition of TOR. Antimicrob Agents Chemother, 2001. 45(11): p. 3162-70.

McCullough, M.J., et al., Species identification and virulence attributes of Saccharomyces boulardii (nom. inval.). J. Clin. Microbiol., 1998. 36(9): p. 2613-2617.

  1. McCullough, M.J., et al., Intergenic transcribed spacer PCR ribotyping for differentiation of Saccharomyces species and interspecific hybrids. J. Clin. Microbiol., 1998. 36: p. 1035-1038.
  2. McCullough, M.J., et al., Epidemiological investigation of vaginal Saccharomyces cerevisiae isolates by a genotypic method. J. Clin. Microbiol., 1998. 36(2): p. 557-562.
  3. Finley, D., et al., Unified nomenclature for subunits of the Saccharomyces cerevisiae proteasome regulatory subunit. Trends Biochem., 1998. 23(7): p. 244-245.
  4. Lashkari, D.A., J.H. McCusker, and R.W. Davis, Whole genome analysis: experimental access to all genome sequenced segments through larger-scale efficient oligonucleotide synthesis and PCR. Proc Natl Acad Sci U S A, 1997. 94(17): p. 8945-7.
  5. Lashkari, D.A., et al., Yeast microarrays for genome wide parallel genetic and gene expression analysis. Proc Natl Acad Sci U S A, 1997. 94(24): p. 13057-62.
  6. Clemons, K.V., et al., Application of DNA typing methods and genetic analysis to epidemiology and taxonomy of Saccharomyces isolates. J Clin Microbiol, 1997. 35(7): p. 1822-8.
  7. Na, S., et al., MOP2 (SLA2) affects the abundance of the plasma membrane H(+)-ATPase of Saccharomyces cerevisiae. J Biol Chem, 1995. 270(12): p. 6815-23.
  1. Winzeler, E.A., et al., Whole genome genetic-typing in yeast using high-density oligonucleotide arrays. Parasitology, 1999. 118 Suppl(Suppl): p. S73-80.
  2. Goldstein, A.L., X. Pan, and J.H. McCusker, Heterologous URA3MX cassettes for gene replacement in Saccharomyces cerevisiae. Yeast, 1999. 15(6): p. 507-11.
  3. Goldstein, A.L. and J.H. McCusker, Three new dominant drug resistance cassettes for gene disruption in Saccharomyces cerevisiae. Yeast, 1999. 15(14): p. 1541-53.

Cruz, M.C., et al., Calcineurin is essential for survival during membrane stress in Candida albicans. Embo J, 2002. 21(4): p. 546-59.

Winzeler, E.A., D. Richards, A. Conway, A.L. Goldstein, S. Kalman, M.J. McCullough, J.H. McCusker, D.A. Stevens, L. Wodicka, D.J. Lockhart, and R.W. Davis. 1998. Direct allelic variation scanning of the yeast genome. Science 281(5380):1194-1197.

Byron, J.K., K.V. Clemons, J.H. McCusker, R.W. Davis, and D.A. Stevens. 1995. Pathogenicity of Saccharomyces cerevisiae in complement factor five (C5) deficient mice. Infec. Immun. 63:478-485.

McCusker, J.H. , K.V. Clemons, D.A. Stevens and R.W. Davis. 1994. Saccharomyces cerevisiae virulence phenotype as determined in CD-1 mice is associated with the ability to grow at 42ºC and form pseudohyphae.Infec. Immun. 62:5447-5455.

McCusker, J.H., K.V. Clemons, D.A. Stevens and R.W. Davis. 1994. Genetic characterization of pathogenicSaccharomyces cerevisiae isolates. Genetics 136:1261-1269.

Clemons, K.V., et al., Comparative pathogenesis of clinical and nonclinical isolates of Saccharomyces cerevisiae. J. Infec. Dis., 1994. 169(4): p. 859-867.

  1. Nelson, S.F., et al., Genomic mismatch scanning: a novel genetic mapping method. Nature Genetics, 1993. 4(1): p. 11-18.
  2. McCusker, J.H., et al., Suppressor analysis of temperature-sensitive RNA polymerase I mutations in Saccharomyces cerevisiae: suppression of mutations in a zinc-binding motif by transposed mutant genes. Mol Cell Biol, 1991. 11(2): p. 746-53.

McCusker, J.H. and R.W. Davis, The use of proline as a nitrogen source causes hypersensitivity to, and allows more economical use of 5FOA in Saccharomyces cerevisiae. Yeast, 1991. 7(6): p. 607-8.

  1. McCusker, J.H. and J.E. Haber, Mutations in Saccharomyces cerevisiae which confer resistance to several amino acid analogs. Mol Cell Biol, 1990. 10(6): p. 2941-2949.
  2. Ramirez, J., et al., ATP sensitive K+ channels in a plasma membrane H+-ATPase mutant of the yeast Saccharomyces cerevisiae. Proc Natl Acad Sci USA, 1989. 86(20): p. 7866-7870.
  3. Balzi, E., et al., The suppressor gene scl1+ of Saccharomyces cerevisiae is essential for growth. Gene, 1989. 83(2): p. 271-279.
  4. McCusker, J.H. and J.E. Haber, Cycloheximide resistant temperature sensitive lethal mutations of Saccharomyces cerevisiae. Genetics, 1988. 119(2): p. 303-315.
  5. McCusker, J.H. and J.E. Haber, crl mutants of Saccharomyces cerevisiae resemble both mutants affecting general control of amino acid biosynthesis and omnipotent translational suppressor mutants. Genetics, 1988. 119(2): p. 317-327.
  6. McCusker, J.H., D.S. Perlin, and J.E. Haber, Pleiotropic plasma membrane ATPase mutations of Saccharomyces cerevisiae. Mol. Cell. Biol., 1987. 7(11): p. 4082-4088.
  7. McCusker, J.H. and J.E. Haber, Evidence of chromosomal breaks near the mating-type locus of Saccharomyces cerevisiae that accompany MATalpha x MATalpha matings. Genetics, 1981. 99: p. 383-403.
  8. Haber, J.E., et al., Chromosomal rearrangements accompanying yeast mating type switching: evidence for a gene conversion model. Cold Spring Harb Symp Quant Biol, 1981. 45 Pt 2: p. 991-1002.