Sriram Vijayraghavan, PhD

McCusker Lab

Postdoctoral Associate
DUMC Box 3020
241 Jones Building
Durham, NC 27710
Phone: 919-681-6778
Fax: 919-684-2790



Research Interest: Research Interest: My research is based on the genetic analysis of quantitative traits in the yeast Saccharomyces cerevisiae. Our lab has sequenced and fully annotated the genomes of over 100 yeast strains that include clinical isolates and multiple non-clinical isolates from diverse geographical locations. My work focuses on analyzing phenotypic variations among these strains under varied growth conditions by high-throughput methods. The current work is aimed at elucidating how discrete genetic changes underlying complex traits influence fitness in challenging environments. Additionally, our work has implications for human health, as the availability of genome-wide phenotype-genotype association data allows us to test the emergence of yeast as an opportunistic pathogen.

Personal Interests: Along with my wife, I greatly enjoy hiking the various state parks in North Carolina.

Publications List:

A Protocol for the Production of Integrase-deficient Lentiviral Vectors for CRISPR/Cas9-mediated Gene Knockout in Dividing Cells.

Vijayraghavan S, Kantor B. J Vis Exp. 2017 Dec 12;(130). doi: 10.3791/56915.

Telomerase Elongation Cycle: Clues from DNA Sequence Variations.

Vijayraghavan S. J Mol Biol. 2017 Jul 7;429(14):2124-2126. doi: 10.1016/j.jmb.2017.05.002. Epub 2017 May 5. No abstract available.

A Checkpoint-Related Function of the MCM Replicative Helicase Is Required to Avert Accumulation of RNA:DNA Hybrids during S-phase and Ensuing DSBs during G2/M.

Vijayraghavan S, Tsai FL, Schwacha A. PLoS Genet. 2016 Aug 24;12(8):e1006277. doi: 10.1371/journal.pgen.1006277. eCollection 2016 Aug.

Mcm2-7 Is an Active Player in the DNA Replication Checkpoint Signaling Cascade via Proposed Modulation of Its DNA Gate.

Tsai FL, Vijayraghavan S, Prinz J, MacAlpine HK, MacAlpine DM, Schwacha A. Mol Cell Biol. 2015 Jun;35(12):2131-43. doi: 10.1128/MCB.01357-14. Epub 2015 Apr 13.

The eukaryotic Mcm2-7 replicative helicase.

Vijayraghavan S, Schwacha A. Subcell Biochem. 2012;62:113-34. doi: 10.1007/978-94-007-4572-8_7. Review.