Bryan Cullen, PhD – Publications

James B. Duke Professor
Founding Director, Center for Virology

researchbiography • lab members • publications • lab website

Google Scholar Profile

Representative Publications:

Zeng, Y., Wagner, E.J., and Cullen, B.R. (2002). Both natural and designed micro RNAs can inhibit the expression of cognate mRNAs when expressed in human cells. Mol Cell, 9:1327-1333.

Bogerd, H.P., Doehle, B.P., Wiegand, H.L., and Cullen, B.R. (2004). A single amino acid difference in the host APOBEC3G protein controls the primate species specificity of HIV type 1 virion infectivity factor. Proc Natl Acad Sci. USA, 101:3770-3774.

Cai, X., Hagedorn, C.H., and Cullen, B.R. (2004).   Human microRNAs are processed from capped, polyadenylated transcripts that can also function as mRNAs. RNA, 10:1957-1966.

Zeng, Y., Yi, R., and Cullen, B.R. (2005). Recognition and cleavage of primary microRNA precursors by the nuclear processing enzyme drosha. EMBO J, 24:138-148.

Cai, X., Lu, S., Zhihong, Z., Gonzalez, C.M., Damania, B., and Cullen, B.R. (2005). Kaposi’s sarcoma-associated herpesvirus expresses an array of novel viral microRNAs in latently infected cells. Proc Natl Acad Sci. USA, 102:5570-5575.

Cai, X., Schäfer, A., Lu, S., Bilello, J.P., Desrosiers, R.C., Edwards, R., Raab-Traub, N., and Cullen, B.R. (2006). Epstein-Barr virus microRNAs are evolutionarily conserved and differentially expressed. PLoS Pathogens, 2:e23.

Bogerd, H.P., Wiegand, H.L., Hulme, A.E., Garcia-Perez, J.L., O’Shea, K.S., Moran, J.V., and Cullen, B.R. (2006). Cellular inhibitors of long interspersed element 1 and Alu retrotransposition. Proc Natl Acad Sci. USA, 103:8780-8785.

Lin, J. and Cullen, B.R. (2007). Analysis of the interaction of primate retroviruses with the human RNA interference machinery. Journal of Virology, 81:12218-12226.

Gottwein, E., Mukherjee, N., Sachse, C., Frenzel, C., Majoros, W.H., Chi, J.-T.A., Braich, R., Manoharan, M., Soutschek, J., Ohler, U., and Cullen, B.R. (2007). A viral microRNA functions as an ortholog of cellular miR-155. Nature, 450:1096-1099.

Umbach, J.L., Kramer, M.F., Jurak, I., Karnowski, H.W., Coen, D.m., and Cullen, B.R. (2008). MicroRNAs expressed by herpes simplex virus 1 during latent infection regulate viral mRNAs. Nature, 454:780-783.

Gottwein, E. and Cullen, B.R. (2008). Viral and cellular microRNAs as determinants of viral pathogenesis and immunity. Cell Host Microbe, 3:375-387.

Cullen, B.R. (2009). Viral and cellular messenger RNA targets of viral microRNAs. Nature, 457:421-425.

Cullen, B.R. (2009). Viral RNAs: Lessons from the enemy. Cell, 136:592-597.

Umbach, J.L. and Cullen, B.R. (2009). The role of RNAi and microRNAs in animal virus replication and antiviral immunity. Genes Dev, 23:1151-1164.

Gottwein, E. and Cullen, B.R. (2010). A human herpesvirus microRNA inhibits p21 expression and attentuates p21-mediated cell cycle arrest. J Virol, 84:5229-5237.

Bogerd, H.P., Karnowski, H.W., Cai, X., Shin, J., Pholers, M., and Cullen, B.R. (2010). A mammalian herpesvirus uses noncanonical expression and processing mechanisms to generate viral MicroRNAs. Mol Cell, 37:135-142.

Bogerd, H.P., Karnowski, H.W., Cai, X., Shin, J., Pholers, M., and Cullen, B.R. (2010). A mammalian herpesvirus uses noncanonical expression and processing mechanisms to generate viral MicroRNAs. Mol Cell, 37:135-142.

Cullen, B.R. (2011). Viruses and microRNAs: RISCy interactions with serious consequences. Genes Dev; 25:1881-1894. PMCID: 3185961.

Gottwein, E., Corcoran, D.L., Mukherjee, N., Skalsky, R.L., Hafner, M., Nusbaum, J.D., Shamulailatpam, P., Love, C.L., Dave, S.S., Tuschl, T., Ohler, U. and Cullen, B.R. (2011). Viral microRNA targetome of KSHV-infected primary effusion lymphoma cell lines. Cell Host Microbe; 10:515-526. PMCID: 3222872.

Skalsky, R.L., Corcoran, D.L., Gottwein, E., Frank, C.L., Kang, D., Hafner, M., Nusbaum, J.D., Feederle, R., Delecluse, H.J., Luftig, M.A., Tuschl, T., Ohler, U. and Cullen, B.R. (2012). The viral and cellular microRNA targetome in lymphoblastoid cell lines. PLoS Pathog; 8:e1002484. PMCID: 3266933.

Feederle, R., Linnstaedt, S.D., Bannert, H., Lips, H., Bencun, M., Cullen, B.R. and Delecluse, H.J. (2011). A viral microRNA cluster strongly potentiates the transforming properties of a human herpesvirus. PLoS Pathog; 7:e1001294. PMCID: 3040666.

Kennedy, Edward M., Bogerd, Hal P., Kornepati, Anand V.R., Kang, Dong, Ghoshal, Delta, Marshall, Joy B., Poling, Brigid C., Tsai, Kevin, Gokhale, Nandan S., Horner, Stacy M. and Cullen, Bryan R. (2016). Posttranscriptional m6A Editing of HIV-1 mRNAs Enhances Viral Gene Expression. Cell Host and Microbe, 19: 675-685

Kennedy, Edward M. and Cullen, Bryan R. (2017) Gene Editing: A New Tool for Viral Disease. Annual Review of Medicine, 68: 401-411.

Kennedy, Edward M., Kornepati, Anand V., Bogerd, Hal P. and Cullen, Bryan R. (2017) Partial reconstitution of the RNAi response in human cells using Drosophila gene products. RNA, 23: 153-160.

Bogerd, Hal P., Kennedy, Edward M., Whisnant, Adam W. and Cullen, Bryan R. (2017) Induced packaging of cellular microRNAs into HIV-1 virions can inhibit infectivity. mBio, 8: e02125-16.

Kennedy, Edward M., Courtney, David G., Tsai, Kevin and Cullen, Bryan R. (2017) Viral epitranscriptomics. Journal of Virology, 91: e02263-16.

Poling, Brigid C., Tsai, Kevin, Kang, Dong, Ren, Linda, Kennedy, Edward M. and Cullen, Bryan R. (2017) A lentiviral vector bearing a reverse intron demonstrates superior expression of both proteins and microRNAs. RNA Biology. 1-10. doi:10.1080/15476286.2017.1334755.

Courtney, David G., Kennedy, Edward M., Dumm, Rebekah E., Bogerd, Hal P., Tsai, Kevin, Heaton, Nicholas S. and Cullen, Bryan R. (2017) Epitranscriptomic enhancement of influenza A virus gene expression and replication. Cell Host & Microbe, 22: 377-386.e5.

Cullen, Bryan R. (2017) RNA interference in mammals: The virus strikes back. Immunity, 46: 970-972.

Poling, Brigid C., Price, Alexander M., Luftig, Micah A. and Cullen, Bryan R. (2017). The Epstein-Barr virus miR-BHRF1 microRNAs regulate viral gene expression in cis. Virology, 512: 113-123.

Heaton, Nicholas S. and Cullen, Bryan R. (2017) Viruses hijack a long non-coding RNA. Nature, 552: 184-185.

Tsai, Kevin, Courtney, David G. and Cullen, Bryan R. (2018) Addition of m6A to SV40 late mRNAs enhances viral structural gene expression and replication. PLoS Pathogens 14: e1006919.