Hal P. Bogerd

Cullen – Lab Members

Headshot of Hal P. Bogerd_Cullen LabResearch Scientist
0045 CARL Building
Box 3025, DUMC
Durham, NC 27710
Phone: (919) 684-5656
Fax: (919) 684-2790

Research Interest: Retrovirology, HIV, molecular virology, miRNAs and CRISPR/cas systems.

Personal Interests: I’m a daily bike commuter and an occasional contributor to the Americana music blog NoDepression.com. I attend more live music than you do. I’ve never worn a fanny pack.

Publications List:

Bogerd HP, Tiley LS, Cullen BR. (1992) Specific binding of the human T-cell leukemia virus type I Rex protein to a short RNA sequence located within the Rex-response element. J Virol; 66: 7572-5.

Bogerd HP, Fridell RA, Blair WS, Cullen BR. (1993) Genetic evidence that the Tat proteins of human immunodeficiency virus types 1 and 2 can multimerize in the eukaryotic cell nucleus. J Virol; 67: 5030-4.

Garrett ED, He F, Bogerd HP, Cullen BR. (1993) Transcriptional trans activators of human and simian foamy viruses contain a small, highly conserved activation domain. J Virol; 67: 6824-7.

Blair WS, Bogerd H, Cullen BR. (1994) Genetic analysis indicates that the human foamy virus Bel-1 protein contains a transcription activation domain of the acidic class. J Virol; 68: 3803-8.

Blair WS, Bogerd HP, Madore SJ, Cullen BR. (1994) Mutational analysis of the transcription activation domain of RelA: identification of a highly synergistic minimal acidic activation module. Mol Cell Biol; 14: 7226-34.

Bogerd HP, Fridell RA, Madore S, Cullen BR. (1995) Identification of a novel cellular cofactor for the Rev/Rex class of retroviral regulatory proteins. Cell; 82: 485-94.

Fridell RA, Harding LS, Bogerd HP, Cullen BR. (1995) Identification of a novel human zinc finger protein that specifically interacts with the activation domain of lentiviral Tat proteins. Virology; 209: 347-57.

Bogerd HP, Fridell RA, Benson RE, Hua J, Cullen BR. (1996) Protein sequence requirements for function of the human T-cell leukemia virus type 1 Rex nuclear export signal delineated by a novel in vivo randomization-selection assay. Mol Cell Biol; 16: 4207-14.

Fridell RA, Benson RE, Hua J, Bogerd HP, Cullen BR. (1996) A nuclear role for the Fragile X mental retardation protein. Embo J; 15: 5408-14.

Fridell RA, Bogerd HP, Cullen BR. (1996) Nuclear export of late HIV-1 mRNAs occurs via a cellular protein export pathway. Proc Natl Acad Sci U S A; 93: 4421-4.

Bieniasz PD, Grdina TA, Bogerd HP, Cullen BR. (1998) Recruitment of a protein complex containing Tat and cyclin T1 to TAR governs the species specificity of HIV-1 Tat. Embo J; 17: 7056-65.

Blair WS, Parsley TB, Bogerd HP, Towner JS, Semler BL, Cullen BR. (1998) Utilization of a mammalian cell-based RNA binding assay to characterize the RNA binding properties of picornavirus 3C proteinases. RNA; 4: 215-25.

Bogerd HP, Echarri A, Ross TM, Cullen BR. (1998) Inhibition of human immunodeficiency virus Rev and human T-cell leukemia virus Rex function, but not Mason-Pfizer monkey virus constitutive transport element activity, by a mutant human nucleoporin targeted to Crm1. J Virol; 72: 8627-35.

Truant R, Fridell RA, Benson RE, Bogerd H, Cullen BR. (1998) Identification and functional characterization of a novel nuclear localization signal present in the yeast Nab2 poly(A)+ RNA binding protein. Mol Cell Biol; 18: 1449-58.

Bieniasz PD, Grdina TA, Bogerd HP, Cullen BR. (1999) Recruitment of cyclin T1/P-TEFb to an HIV type 1 long terminal repeat promoter proximal RNA target is both necessary and sufficient for full activation of transcription. Proc Natl Acad Sci U S A; 96: 7791-6.

Bieniasz PD, Grdina TA, Bogerd HP, Cullen BR. (1999) Highly divergent lentiviral Tat proteins activate viral gene expression by a common mechanism. Mol Cell Biol; 19: 4592-9.

Bieniasz PD, Grdina TA, Bogerd HP, Cullen BR. (1999) Analysis of the effect of natural sequence variation in Tat and in cyclin T on the formation and RNA binding properties of Tat-cyclin T complexes. J Virol; 73: 5777-86.

Bogerd HP, Benson RE, Truant R, Herold A, Phingbodhipakkiya M, Cullen BR. (1999) Definition of a consensus transportin-specific nucleocytoplasmic transport signal. J Biol Chem; 274: 9771-7.

Kang Y, Bogerd HP, Yang J, Cullen BR. (1999) Analysis of the RNA binding specificity of the human tap protein, a constitutive transport element-specific nuclear RNA export factor. Virology; 262: 200-9.

Yang J, Bogerd HP, Peng S, Wiegand H, Truant R, Cullen BR. (1999) An ancient family of human endogenous retroviruses encodes a functional homolog of the HIV-1 Rev protein. Proc Natl Acad Sci U S A; 96: 13404-8. PMCID: PMC23960.

Bogerd HP, Wiegand HL, Bieniasz PD, Cullen BR. (2000) Functional differences between human and bovine immunodeficiency virus Tat transcription factors. J Virol; 74: 4666-71. PMCID: PMC111987.

Bogerd HP, Wiegand HL, Yang J, Cullen BR. (2000) Mutational definition of functional domains within the Rev homolog encoded by human endogenous retrovirus K. J Virol; 74: 9353-61. PMCID: PMC112363.

Kang Y, Bogerd HP, Cullen BR. (2000) Analysis of cellular factors that mediate nuclear export of RNAs bearing the Mason-Pfizer monkey virus constitutive transport element. J Virol; 74: 5863-71. PMCID: PMC112081.

Neufeld KL, Nix DA, Bogerd H, Kang Y, Beckerle MC, Cullen BR, White RL. (2000) Adenomatous polyposis coli protein contains two nuclear export signals and shuttles between the nucleus and cytoplasm. Proc Natl Acad Sci U S A; 97: 12085-90. PMCID: PMC17298.

Yang J, Bogerd H, Le SY, Cullen BR. (2000) The human endogenous retrovirus K Rev response element coincides with a predicted RNA folding region. RNA; 6: 1551-64. PMCID: PMC1370025.

Yang J, Bogerd HP, Wang PJ, Page DC, Cullen BR. (2001) Two closely related human nuclear export factors utilize entirely distinct export pathways. Mol Cell; 8: 397-406.

Wiegand HL, Coburn GA, Zeng Y, Kang Y, Bogerd HP, Cullen BR. (2002) Formation of Tap/NXT1 heterodimers activates Tap-dependent nuclear mRNA export by enhancing recruitment to nuclear pore complexes. Mol Cell Biol; 22: 245-56. PMCID: PMC134221.

Yi R, Bogerd HP, Cullen BR. (2002) Recruitment of the Crm1 nuclear export factor is sufficient to induce cytoplasmic expression of incompletely spliced human immunodeficiency virus mRNAs. J Virol; 76: 2036-42. PMCID: PMC153812.

Yi R, Bogerd HP, Wiegand HL, Cullen BR. (2002) Both ran and importins have the ability to function as nuclear mRNA export factors. RNA; 8: 180-7. PMCID: PMC1370241.

Bogerd HP, Doehle BP, Wiegand HL, Cullen BR. (2004) A single amino acid difference in the host APOBEC3G protein controls the primate species specificity of HIV type 1 virion infectivity factor. Proc Natl Acad Sci U S A; 101: 3770-4. PMCID: PMC374319.

Schäfer A, Bogerd HP, Cullen BR. (2004) Specific packaging of APOBEC3G into HIV-1 virions is mediated by the nucleocapsid domain of the gag polyprotein precursor. Virology; 328: 163-8.

Wiegand HL, Doehle BP, Bogerd HP, Cullen BR. (2004) A second human antiretroviral factor, APOBEC3F, is suppressed by the HIV-1 and HIV-2 Vif proteins. EMBO J; 23: 2451-8. PMCID: PMCPMC423288.

Doehle BP, Schäfer A, Wiegand HL, Bogerd HP, Cullen BR. (2005) Differential sensitivity of murine leukemia virus to APOBEC3-mediated inhibition is governed by virion exclusion. J Virol; 79: 8201-7. PMCID: PMC1143768.

Bogerd HP, Wiegand HL, Doehle BP, Lueders KK, Cullen BR. (2006) APOBEC3A and APOBEC3B are potent inhibitors of LTR-retrotransposon function in human cells. Nucleic Acids Res; 34: 89-95. PMCID: PMC1326241.

Bogerd HP, Wiegand HL, Hulme AE, Garcia-Perez JL, O’Shea KS, Moran JV, Cullen BR. (2006) Cellular inhibitors of long interspersed element 1 and Alu retrotransposition. Proc Natl Acad Sci U S A; 103: 8780-5. PMCID: PMC1482655.

Doehle BP, Bogerd HP, Wiegand HL, Jouvenet N, Bieniasz PD, Hunter E, Cullen BR. (2006) The betaretrovirus Mason-Pfizer monkey virus selectively excludes simian APOBEC3G from virion particles. J Virol; 80: 12102-8. PMCID: PMC1676301.

Bogerd HP, Wiegand HL, Doehle BP, Cullen BR. (2007) The intrinsic antiretroviral factor APOBEC3B contains two enzymatically active cytidine deaminase domains. Virology; 364: 486-93. PMCID: PMC1950718.

Hulme AE, Bogerd HP, Cullen BR, Moran JV. (2007) Selective inhibition of Alu retrotransposition by APOBEC3G. Gene; 390: 199-205.

Bogerd HP, Cullen BR. (2008) Single-stranded RNA facilitates nucleocapsid: APOBEC3G complex formation. RNA; 14: 1228-36. PMCID: PMC2390788.

Bogerd HP, Tallmadge RL, Oaks JL, Carpenter S, Cullen BR. (2008) Equine infectious anemia virus resists the anti-retroviral activity of equine APOBEC3 proteins through a packaging-independent mechanism. J Virol; 82: 11889-901. PMCID: PMC2583693.

Bogerd HP, Karnowski HW, Cai X, Shin J, Pohlers M, Cullen BR. (2010) A mammalian herpesvirus uses noncanonical expression and processing mechanisms to generate viral microRNAs. Mol Cell; 37: 135-42. PMCID: PMC2818755.

Bogerd HP, Zhang F, Bieniasz PD, Cullen BR. (2011) Human APOBEC3 proteins can inhibit xenotropic murine leukemia virus-related virus infectivity. Virology; 410: 234-9. PMCID: PMC3035163.

Zhang A, Bogerd H, Villinger F, Gupta JD, Dong B, Klein EA, Hackett J, Jr., Schochetman G, Cullen BR, Silverman RH. (2011) In vivo hypermutation of xenotropic murine leukemia virus-related virus DNA in peripheral blood mononuclear cells of rhesus macaque by APOBEC3 proteins. Virology; 421: 28-33. PMCID: PMC3208790.

Whisnant AW, Bogerd HP, Flores O, Ho P, Powers JG, Sharova N, Stevenson M, Chen CH, Cullen BR. (2013) In-depth analysis of the interaction of HIV-1 with cellular microRNA biogenesis and effector mechanisms. mBio; 4: e000193. PMCID: PMC3634607.

Bogerd HP, Whisnant AW, Kennedy EM, Flores O, Cullen BR. (2014) Derivation and characterization of Dicer- and microRNA-deficient human cells. RNA; 20: 923-37. PMCID: PMC4024645.

Bogerd HP, Skalsky RL, Kennedy EM, Furuse Y, Whisnant AW, Flores O, Schultz KL, Putnam N, Barrows NJ, Sherry B, Scholle F, Garcia-Blanco MA, Griffin DE, Cullen BR. (2014) Replication of many human viruses is refractory to inhibition by endogenous cellular microRNAs. J Virol; 88: 8065-76. PMCID: PMC4097787.

Kennedy EM, Kornepati AV, Goldstein M, Bogerd HP, Poling BC, Whisnant AW, Kastan MB, Cullen BR. (2014) Inactivation of the human papillomavirus E6 or E7 gene in cervical carcinoma cells by using a bacterial CRISPR/Cas RNA-guided endonuclease. J Virol; 88: 11965-72. PMCID: PMC4178730.

Kennedy EM, Bassit LC, Mueller H, Kornepati AVR, Bogerd HP, Nie T, Chatterjee P, Javanbakht H, Schinazi RF, Cullen BR. (2015) Suppression of hepatitis B virus DNA accumulation in chronically infected cells using a bacterial CRISPR/Cas RNA-guided DNA endonuclease. Virology; 476: 196-205. PMCID: PMC4323668.

Mefferd AL, Kornepati AV, Bogerd HP, Kennedy EM, Cullen BR. (2015) Expression of CRISPR/Cas single guide RNAs using small tRNA promoters. RNA; 21: 1683-9. PMCID: PMC4536327.

Bogerd HP, Kornepati AV, Marshall JB, Kennedy EM, Cullen BR. (2015) Specific induction of endogenous viral restriction factors using CRISPR/Cas-derived transcriptional activators. Proc Natl Acad Sci U S A; 112: E7249-56. PMCID: PMC4703010.

Kennedy EM, Whisnant AW, Kornepati AV, Marshall JB, Bogerd HP, Cullen BR. (2015) Production of functional small interfering RNAs by an amino-terminal deletion mutant of human Dicer. Proc Natl Acad Sci U S A; 112: E6945-54. PMCID: PMC4687602.

Kennedy EM, Kornepati AV, Mefferd AL, Marshall JB, Tsai K, Bogerd HP, Cullen BR. (2015) Optimization of a multiplex CRISPR/Cas system for use as an antiviral therapeutic. Methods; 91: 82-6. PMCID: PMC4684739.