David Courtney, PhD

Cullen – Lab Members

Headshot of David CourtneyResearch Scholar
0045 CARL Building
DUMC
Durham, NC 27710
Phone: 684-565-0045
Fax: 919-684-2790
Email: david.courtney@duke.edu

Research Interest: My PhD research focused on gene therapies, specifically utilizing siRNA and CRISPR/Cas9 systems. These therapies were primarily targeted to autosomal dominant corneal dystrophies. However, since completing my PhD I have shifted my research interests to virology with a primary focus on the role of RNA methylation in viral pathogenesis, and secondly on the development of CRISPR/Cas9 viral vectors to treat both genetic disorders and viral infections.

Personal Interests: Outside of the lab I have a keen interest in traveling, and having grown up in Ireland and travelled over Europe I am looking forward to seeing more of the Americas during my Postdoc here at Duke. I am also a big football fan, wholeheartedly supporting the Green Bay Packers on Sunday afternoons, while also getting up early to watch the soccer back home, namely Manchester United.

Publications List:

  1. Courtney, D.G., Allen, E.H.A., Atkinson, S.D., et al. 2016 Keratin 12 missense mutation induces the unfolded protein response and apoptosis in Meesmann epithelial corneal dystrophy. Hum Mol Genet. doi: 10.1093/hmg/ddw001
  2. Courtney, D.G., Thakur, A., Nesbit, M.A., et al., 2015. A review of personalised molecular medicine for the treatment of corneal disorders. Int J Ophthalmol Eye Res. S2:001, 7-18.
  3. Courtney, D.G., Moore, J.E., Atkinson, S.D., et al., 2015. CRISPR/Cas9 DNA cleavage at SNP-derived PAM enables both in vitro and in vivo KRT12 mutation-specific targeting. Gene Ther. Published Online First: 20 August 2015. doi:10.1038/gt.2015.82.
  4. Courtney, D.G., Toftgaard Poulsen, E., Kennedy, S., et al., 2015. Protein Composition of TGFBI-R124C- and TGFBI-R555W- Associated Aggregates Suggests Multiple Mechanisms Leading to Lattice and Granular Corneal Dystrophy. Invest. Ophthalmol. Vis. Sci. 56, 4653–4661.
  5. Courtney, D.G., Atkinson, S.D., Allen, E.H.A., et al., 2014. siRNA Silencing of the Mutant Keratin 12 Allele in Corneal Limbal Epithelial Cells Grown from Patients with Meesmann’s Epithelial Corneal Dystrophy. Invest. Ophthalmol. Vis. Sci. 55, 3352–60.
  6. Courtney, D.G., Atkinson, S.D., Moore, J.E., et al., 2014. Development of allele specific gene silencing siRNAs for TGFBI Arg124Cys in Lattice Corneal Dystrophy Type I. Invest. Ophthalmol. Vis. Sci. 55, 977-985.
  7. Moore, J.E., Atkinson, S.D., Azar, D.T., Worthington, J., Downes, C.S., Courtney, D.G., Moore, C.B.T., 2014. Protection of corneal epithelial stem cells prevents ultraviolet A damage during corneal collagen cross-linking treatment for keratoconus. Br. J. Ophthalmol. 98, 270–4.