David Courtney, PhD
0045 CARL Building
Durham, NC 27710
Research Interest: My PhD research focused on gene therapies, specifically utilizing siRNA and CRISPR/Cas9 systems. These therapies were primarily targeted to autosomal dominant corneal dystrophies. However, since completing my PhD I have shifted my research interests to virology with a primary focus on the role of RNA methylation in viral pathogenesis, and secondly on the development of CRISPR/Cas9 viral vectors to treat both genetic disorders and viral infections.
Personal Interests: Outside of the lab I have a keen interest in traveling, and having grown up in Ireland and travelled over Europe I am looking forward to seeing more of the Americas during my Postdoc here at Duke. I am also a big football fan, wholeheartedly supporting the Green Bay Packers on Sunday afternoons, while also getting up early to watch the soccer back home, namely Manchester United.
- Courtney, D.G., Allen, E.H.A., Atkinson, S.D., et al. 2016 Keratin 12 missense mutation induces the unfolded protein response and apoptosis in Meesmann epithelial corneal dystrophy. Hum Mol Genet. doi: 10.1093/hmg/ddw001
- Courtney, D.G., Thakur, A., Nesbit, M.A., et al., 2015. A review of personalised molecular medicine for the treatment of corneal disorders. Int J Ophthalmol Eye Res. S2:001, 7-18.
- Courtney, D.G., Moore, J.E., Atkinson, S.D., et al., 2015. CRISPR/Cas9 DNA cleavage at SNP-derived PAM enables both in vitro and in vivo KRT12 mutation-specific targeting. Gene Ther. Published Online First: 20 August 2015. doi:10.1038/gt.2015.82.
- Courtney, D.G., Toftgaard Poulsen, E., Kennedy, S., et al., 2015. Protein Composition of TGFBI-R124C- and TGFBI-R555W- Associated Aggregates Suggests Multiple Mechanisms Leading to Lattice and Granular Corneal Dystrophy. Invest. Ophthalmol. Vis. Sci. 56, 4653–4661.
- Courtney, D.G., Atkinson, S.D., Allen, E.H.A., et al., 2014. siRNA Silencing of the Mutant Keratin 12 Allele in Corneal Limbal Epithelial Cells Grown from Patients with Meesmann’s Epithelial Corneal Dystrophy. Invest. Ophthalmol. Vis. Sci. 55, 3352–60.
- Courtney, D.G., Atkinson, S.D., Moore, J.E., et al., 2014. Development of allele specific gene silencing siRNAs for TGFBI Arg124Cys in Lattice Corneal Dystrophy Type I. Invest. Ophthalmol. Vis. Sci. 55, 977-985.
- Moore, J.E., Atkinson, S.D., Azar, D.T., Worthington, J., Downes, C.S., Courtney, D.G., Moore, C.B.T., 2014. Protection of corneal epithelial stem cells prevents ultraviolet A damage during corneal collagen cross-linking treatment for keratoconus. Br. J. Ophthalmol. 98, 270–4.