Duke University Medical Center
Faculty and Research

John Perfect, MD
James B. Duke Professor
Department of Medicine, Division of Infectious Diseases
Chief, Division of Infectious Diseases
Director, Duke University Mycology Research Unit (DUMRU)
biography  •  lab members  

Selected Publications:

  • Hu G, Cheng PY, Sham A, Perfect JR, Kronstad JW. Metabolic adaptation in Cryptococcus neoformans during early murine pulmonary infection. Mol Microbiol. 2008 Sep;69(6):1456-75. Epub 2008 Jul 30.
  • Segal BH, Herbrecht R, Stevens DA, Ostrosky-Zeichner L, Sobel J, Viscoli C, Walsh TJ, Maertens J, Patterson TF, Perfect JR, Dupont B, Wingard JR, Calandra T, Kauffman CA, Graybill JR, Baden LR, Pappas PG, Bennett JE, Kontoyiannis DP, Cordonnier C, Viviani MA, Bille J, Almyroudis NG, Wheat LJ, Graninger W, Bow EJ, Holland SM, Kullberg BJ, Dismukes WE, De Pauw BE. Defining responses to therapy and study outcomes in clinical trials of invasive fungal diseases: Mycoses Study Group and European Organization for Research and Treatment of Cancer consensus criteria. Clin Infect Dis. 2008 Sep 1;47(5):674-83.
  • Baddley JW, Perfect JR, Oster RA, Larsen RA, Pankey GA, Henderson H, Haas DW, Kauffman CA, Patel R, Zaas AK, Pappas PG. Pulmonary cryptococcosis in patients without HIV infection: Factors associated with disseminated disease. Eur J Clin Microbiol Infect Dis. 2008 Oct;27(10):937-43. Epub 2008 May 1.
  • Giles SS, Zaas AK, Reidy MF, Perfect JR, Wright JR. Cryptococcus neoformans is resistant to surfactant protein A mediated host defense mechanisms. PLoS ONE. 2007 Dec 26;2(12):e1370.
  • Chayakulkeeree M, Rude TH, Toffaletti DL, Perfect JR. Fatty acid synthesis is essential for survival of Cryptococcus neoformans and a potential fungicidal target. Antimicrob Agents Chemother. 2007 Oct;51(10):3537-45. Epub 2007 Aug 13.
  • Steinbach WJ, Reedy JL, Cramer RA Jr, Perfect JR, Heitman J. Harnessing calcineurin as a novel anti-infective agent against invasive fungal infections. Nat Rev Microbiol. 2007 Jun;5(6):418-30. Review.
  • Wormley FL Jr, Perfect JR, Steele C, Cox GM. Protection against cryptococcosis by using a murine gamma interferon-producing Cryptococcus neoformans strain. Infect Immun. 2007 Mar;75(3):1453-62. Epub 2007 Jan 8.
  • Idnurm A, Giles SS, Perfect JR, Heitman J. Peroxisome function regulates growth on glucose in the basidiomycete fungus Cryptococcus neoformans. Eukaryot Cell. 2007 Jan;6(1):60-72. Epub 2006 Oct 13.
  • Petzold EW, Himmelreich U, Mylonakis E, Rude T, Toffaletti D, Cox GM, Miller JL, Perfect JR. Characterization and regulation of the trehalose synthesis pathway and its importance in the pathogenicity of Cryptococcus neoformans. Infect Immun. 2006 Oct;74(10):5877-87.
  • Chayakulkeeree M, Perfect JR. Cryptococcosis. Infect Dis Clin North Am. 2006 Sep;20(3):507-44, v-vi. Review.
  • Giles SS, Stajich JE, Nichols C, Gerrald QD, Alspaugh JA, Dietrich F, Perfect JR. The Cryptococcus neoformans catalase gene family and its role in antioxidant defense. Eukaryot Cell. 2006 Sep;5(9):1447-59.
  • Perfect JR. Cryptococcus neoformans: The yeast that likes it hot. FEMS Yeast Res. 2006 Jun;6(4):463-8. Review.
  • Bahn YS, Cox GM, Perfect JR, Heitman J. Carbonic anhydrase and CO2 sensing during Cryptococcus neoformans growth, differentiation, and virulence. Curr Biol. 2005 Nov 22;15(22):2013-20. PMID: 16303560
  • Perfect JR. Cryptococcus neoformans: A sugar-coated killer with designer genes. FEMS Immunol Med Microbiol. 2005 Sep 1;45(3):395-404. Review.
  • Wormley FL Jr, Heinrich G, Miller JL, Perfect JR, Cox GM. Identification and characterization of an SKN7 homologue in Cryptococcus neoformans. Infect Immun. 2005 Aug;73(8):5022-30.
  • Nielsen K, Cox GM, Litvintseva AP, Mylonakis E, Malliaris SD, Benjamin DK Jr, Giles SS, Mitchell TG, Casadevall A, Perfect JR, Heitman J. Cryptococcus neoformans {alpha} strains preferentially disseminate to the central nervous system during coinfection. Infect Immun. 2005 Aug;73(8):4922-33.
  • Wormley FL Jr, Perfect JR. Immunology of infection caused by Cryptococcus neoformans. Methods Mol Med. 2005 118:193-8.
  • Wormley FL Jr, Cox GM, Perfect JR. Evaluation of host immune responses to pulmonary cryptococcosis using a temperature-sensitive C. neoformans calcineurin A mutant strain. Microb Pathog. 2005 Feb-Mar;38(2-3):113-23.
  • Giles SS, Batinic-Haberle I, Perfect JR, Cox GM. Cryptococcus neoformans mitochondrial superoxide dismutase: an essential link between antioxidant function and high-temperature growth. Eukaryot Cell. 2005 Jan;4(1):46-54.
  • Giles SS, Perfect JR, Cox GM. Cytochrome c peroxidase contributes to the antioxidant defense of Cryptococcus neoformans. Fungal Genet Biol. 2005 Jan;42(1):20-9.

  • Perfect, JR. Nuances of new anti-Aspergillus antifungals. Med Mycol. 2005 1:S271-276.
  • Perfect JR. Posaconazole: A viewpoint by John R. Perfect. Drugs 2005 65:1568-1569.

  • Chang CY, Schell WA, Perfect JR, and Hulka GF. Novel use of a swimming pool biocide in the treatment of a rare fungal mastoiditis. Laryngoscope 2005 115:1065-1069.

  • Gibbs WJ, Drew RH, and Perfect JR. Liposomal amphotericin B: Clinical experience and perspectives. Expert Rev Anti Infect Ther. 2005 3:167-181.
  • Drew RH, Arthur RR, and Perfect JR. Is it time to abandon the use of amphotericin B bladder irrigation? Clin Infect Dis. 2005 40:1465-1470.

  • Venkatesan P, Perfect JR, and Myers SA. Evaluation and management of fungal infections in immunocompromised patients. Dermatol Ther. 2005 18:44-57.

  • Baddour LM, Perfect JR, Ostrosky-Zeichner L. Successful use of amphotericin B lipid complex in the treatment of cryptococcosis. 2005 Clin Infect Dis. 6:S409-13.

  • Perfect JR. Treatment of non-Aspergilus moulds in immunocompromised patients, with amphotericin B lipid complex. Clin Infect Dis. 6:S401-408, 2005.
  • Perfect JR. Antifungal resistance: The clinical front. Oncology (Williston Park). 2004 18:15-22.

  • Perfect JR. Use of newer antifungal therapies in clinical practice: What do the data tell us? Oncology (Williston Park). 2004 18:15-23.
  • Walsh T, Pappas P, Winston D, Blumer J, Peterson F, Yanovich S, Raffalli J, Stiff P, Greenberg R, Donowitz G, Lee J, Wingard J, Fioritoni G, Finberg R, Laverdiere M, Perfect JR, Schuster M, Arndt C, Reinhardt J, Reboli A, Garber SG, Lynch JR, Ricci P, Anaissie E. Voriconazole compared with liposomal amphotericin B for empiric therapy in patients with neutropenia and persistent fever. N. Engl. J. Med. 2002 34:225-234.
  • Cox GM, Durack DT, and Perfect J. Fungal infections and the central nervous system (ed MJ Aminoff). Neurology and General Medicine 2001 3rd ed., pp 789-807.

  • Luberto C, Toffaletti DL, Wills EA, Tucker S, Casadevall A, Perfect JR, Hannun YA, Del Poeta M. Roles for inositol-phosphoryl ceramide 1 (IPC1) in pathogenesis of Cryptococcus neoformans. Genes and Dev. 2001 15:201-212.

  • Wills EA, Roberts IS, Del Poeta M, Cox GM, Rivera J, Casadevall A, Perfect JR. Identification and characterization of the Cryptococcus neoformans phosphomannose isomerase-encoding gene, MAN1, and its impact on pathogenicity. Mol. Microbiol. 2001 40:1-13.

  • D'Souza C, Alspaugh JA, Yue C, Cox GM, Perfect JR, Heitman J. cAMP dependent protein kinase controls virulence of the fungal pathogen, Cryptococcus neoformans. Mol. Cell. Biol. 2001 21:3179-3191.

  • Stephens CE, Tanious F, Wilson DW, Schell WA, Perfect JR, Fransblau SG, Boykin DW. Diguanidino and "reversed" diamidino 2,5-diarylfurans as antimicrobial agents. J. Med. Chem. 2001 44:1741-1748.