Duke University Medical Center
DUKE MYCOLOGY RESEARCH UNIT
Faculty and Research

J. Andrew Alspaugh, MD
Professor
Department of Medicine, Division of Infectious Diseases
research  •  biography  •  publications  •  lab members  • website

Selected Publications:

C Skrynia, DM Binninger, JA Alspaugh and PJ Pukkila.  Molecular characterization of TRP1, a gene encoding for tryptophan synthetase in the basidiomycete Coprinus cinereus. Gene 81:73-82, 1989. PMID 2806911.

JA Alspaugh and DL Granger. Inhibition of Cryptococcus neoformans replication by nitrogen oxides supports the role of these molecules as effectors of macrophage-mediated cytostasis. Infect. Immun. 59:2291-2296, 1991. PMID 2050398.

JA Alspaugh, JR Perfect, and J Heitman. Cryptococcus neoformans mating and virulence are regulated by the G-protein a subunit GPA1 and cAMP. Genes & Development 11:3206-3217, 1997. PMID 9389652.

JA Alspaugh and JR Perfect. Infections due to zygomycetes and other rare opportunistic fungi. Seminars in Respiratory and Critical Care Medicine 18:265-279, 1997.

GM Lyons, JA Alspaugh, FT Meredith, LJ Harrell, V Tapson, RD Davis, and SS Kanj. Mycoplasma hominis pneumonia complicating bilateral lung transplantation: Case report and review of the literature. Chest 112: 1428-1432, 1997. PMID 9367488.

MA Mallory, RE Nettles, A Alspaugh, and DJ Sexton. Community acquired prosthetic valve endocarditis due to methicillin-resistant S. aureus. Clin Infect Dis 24:1272-1273, 1997.

VG Fowler Jr, FM Nacinovich, JA Alspaugh, and GR Corey. Prosthetic joint infection with Histoplasma capsulatum: case report and review of the literature. Clin Infect Dis 26:1017, 1998. PMID 9564509.

JA Alspaugh, JR Perfect, and J Heitman. Signal transduction pathways regulating differentiation and pathogenicity of Cryptococcus neoformans. Fungal Genet Biol 25:1-14, 1998. PMID 9806801.

S Sudarshan, RC Davidson, J Heitman, and JA Alspaugh. Molecular characterization of the Cryptococcus neoformans ADE2 gene, a selectable marker for transformation and gene disruption. Fungal Genet Biol 1999, 27: 36-48. 1999. PMID 10413613.

C Yue, LM Cavallo, JA Alspaugh, P Wang, GM Cox, JR Perfect, and J Heitman. The STE12a homolog is required for haploid filamentation but largely dispensable for mating and virulence in Cryptococcus neoformans, Genetics 153: 1601-1615, 1999. PMID 10581270.

J Heitman, B Allen, JA Alspaugh, KJ Kwon-Chung. On the origins of congenic MATalpha and MATa strains of the pathogenic yeast Cryptococcus neoformans. Fungal Genet Biol 28: 1-5, 1999. PMID 10512666.

RC Davidson, MC Cruz, RA Sia, BM Allen, JA Alspaugh, and J Heitman. Gene disruption by biolistic transformation in serotype D strains of Cryptococcus neoformans. Fungal Genet Biol 29: 38-48, 2000. PMID 10779398.

JA Alspaugh, LM Cavallo, JR Perfect and J Heitman. RAS1 regulates filamentation, mating and growth at high temperature of Cryptococcus neoformans. Molecular Microbiology 36: 352-365, 2000. PMID 10792722.

JA Alspaugh, RC Davidson, and J Heitman. Morphogenesis in Cryptococcus neoformans. In J. F. Ernst and A. Schmidt, eds. Dimorphism in Human Pathogenic and Apathogenic Yeasts. Karger: Basel, 2000. PMID 10863675.

CA D’Souza, JA Alspaugh, C Yue, T Harashima, GM Cox, JR Perfect, and J Heitman. Cyclic AMP-dependent protein kinase controls virulence of the fungal pathogen Cryptococcus neoformans. Molecular and Cellular Biology 21:3179-3191, 2001. PMID 11287622.

JA Alspaugh, R Pukkila-Worley, T Harashima, LM Cavallo, D Funnell, GM Cox, JR Perfect, JW Kronstad, and J Heitman. Adenylyl cyclase functions downstream of the G alpha protein GPA1 and controls mating and pathogenicity of Cryptococcus neoformans, Eukaryotic Cell 1:75-84, 2002. PMID 12455973.

MS Waugh, CS Breeding, CM DeCesare, GM Cox, J Heitman, and JA Alspaugh. Ras1 and Ras2 contribute shared and unique roles in physiology and virulence of Cryptococcus neoformans. Microbiology 148: 191-201, 2002. PMID 11782511.

D Schiappa, A Geuyikian, S Kakar, JA Alspaugh, JR Perfect, and PR Williamson. An auxotrophic pigmented Cryptococcus neoformans strain causing infection of the bone marrow. Medical Mycology 40: 1-5, 2002. PMID 11860008.

Z Yang, JA Alspaugh, GM Cox, JM McCusker. Requirement of the C. neoformans MET3 gene for growth and pathogenicity, Microbiology 148:2617-2675, 2002. PMID 12177356.

JA Alspaugh and JR Perfect. Cryptococcal Meningitis. Current Treatment Options in Infectious Diseases 4:75-80, 2002.

MS Waugh, MA Vallim, J Heitman, JA Alspaugh. Ras1 controls pheromone expression and response during mating in C. neoformans. Fungal Genetics and Biology 38:110-121, 2003. PMID 12553941.

R Pukkila-Worley and JA Alspaugh. Cyclic AMP signaling in Cryptococcus neoformans. FEMS Yeast Research 4:361-367, 2004. PMID 14734016.

MA Vallim, L Fernandes, and JA Alspaugh. The RAM1 gene encoding a protein-farnesyltransferase beta-subunit homolog is essential in Cryptococcus neoformans. Microbiology, 150:1925-1935, 2004. PMID 15184578.

R Pukkila-Worley, QD Gerrald, PR Kraus, M-J Boily, MJ Davis, SS Giles, GM Cox, J Heitman, and JA Alspaugh. Transcriptional network of multiple capsule and melanin genes governed by the C. neoformans cAMP cascade. Eukaryotic Cell 4:190-201, 2005. PMID 15643074.

JA Fraser, JC Huang, R. Pukkila-Worley, JA Alspaugh, TG Mitchell, and J. Heitman. Sex-induced chromosomal translocation and segmental duplication in Cryptococcus neoformans. Eukaryotic Cell 4: 401-406, 2005. PMID 15701802.

MA Vallim, CB Nichols, L Fernandes, K Cramer, and JA Alspaugh. A Rac homolog functions downstream of Ras1 to control hyphal differentiation and high temperature growth in the pathogenic fungus C. neoformans. Eukaryotic Cell 4: 1066-1078, 2005. PMID 15947199.

JA Alspaugh and JR Perfect. “Fungal meningitis”. In Roos KL, ed. Principles of Neurologic Infectious Diseases, New York: McGraw-Hill, 2005.

BD Alexander, ES Dodds-Ashley, RM Addison, JA Alspaugh, NJ Chao, JR Perfect. Non-comparative evaluation of the safety of aerosolized amphotericin B lipid complex in patients undergoing allogeneic hematopoietic stem cell transplantation. Transplant Infectious Dis 8:13-20, 2006. PMID 16623816.

K Cramer, QD Gerrald, CB Nichols, MS Price, and JA Alspaugh. The transcription factor Nrg1 mediates capsule, stress response, and pathogenesis in Cryptococcus neoformans. Eukaryotic Cell 5:1147-1156, 2006. PMID 16835458.

SS Giles, JE Stajich, QD Gerrald, CB Nichols, JA Alspaugh, JR Perfect. The C. neoformans catalase gene family and its role in antioxidant defense. Eukaryotic Cell 5: 1447-1459, 2006. PMID 16963629.

GM Olson, DS Fox, P Wang, JA Alspaugh, KL Buchanan. The role of protein O-mannosyltransferase Pmt4 in the morphogenesis and virulence of Cryptococcus neoformans. Eukaryotic Cell, 5:1447-1459, 2006. PMID 17142566.

CB Nichols, Z Perfect, JA Alspaugh. A Ras1-Cdc24 signal transduction pathway mediates thermotolerance in the fungal pathogen C. neoformans. Molecular Microbiology 63: 1118-1130, 2007. PMID 17233829.

JA Alspaugh. Immunotherapy in fungal infections. J Invasive Fungal Infect 2: 4-10, 2008.

MS Price, CB Nichols, JA Alspaugh. Cryptococcus neoformans Rho-GDP dissociation inhibitor mediates intracellular survival and virulence. Infection and Immunity, 76: 5729-5737, 2008. PMID: 18779335. PMCID: PMC2583580

JR Fortwendel, PR Juvvadi, N Pinchai, BZ Perfect, JA Alspaugh, JR Perfect, and WJ Steinbach Differential effects of chitin and 1,3-b-D-glucan synthesis Inhibition on Ras and calcineurin mutants in Aspergillus fumigatus, Antimicrobial Agents and Chemotherapy 53:476-82, 2009, PMID: 19015336. PMCID: PMC2630655

CB Nichols, J Ferreyra, ER Ballou, JA Alspaugh. Subcellular localization directs signaling specificity of the Cryptococcus neoformans Ras1 protein. Eukaryotic Cell 8:181-189, 2009. PMID 19098128. PMCID: PMC2643607

SD Willger, JF Ernst, JA Alspaugh, KB Lengeler. Characterization of the PMT gene family in C. neoformans. PLoS One, 4(7): e6321. doi:10.1371/journal.pone.0006321, 2009. PMID 19633715. PMCID: PMC2711527

E Ballou, CB Nichols, L Kozubowski, K Miglia, JA Alspaugh. Two CDC42 paralogs modulate C. neoformans thermotolerance and morphogenesis under host physiological conditions. Molecular Microbiology 75:763-780, 2010, PMID 20025659. PMCID: PMC3685590

TR O’Meara, D Norton, MS Price, C Hay, MF Clements, CB Nichols, JA Alspaugh. Interaction of C. neoformans Rim101 and Protein Kinase A regulates capsule. PLoS Pathogens 6(2): e1000776. doi:10.1371/journal.ppat.10000776, 2010. PMCID: PMC2824755

TR O’Meara, C Hay, MS Price, SS Giles, JA Alspaugh. Cryptococcus neoformans histone acetyltransferase Gcn5 regulates fungal adaptation to the host. Eukaryotic Cell, 9: 1103-1202, 2010, PMID 20581290. PMCID: PMC2918934

JA Alspaugh, CB Nichols, C Yue, W-C Shen, and P Wang. “G-protein signaling pathways regulating morphogenesis and virulence of Cryptococcus neoformans”. In Heitman J, et al., ed. Cryptococcus: from human pathogen to model yeast, Philadelphia: ASM Press, 2010.

WA Schell, K O’Donnell, JA Alspaugh. Heterothallic Mating in Mucor irregularis and first Isolate of the species outside of Asia. Medical Mycology 49: 714-723, 2011, PMID 2145328.

MA Hast, CB Nichols, SM Armstrong, SM Kelly, HW Hellinga, JA Alspaugh, LS Beese. Structures of Cryptococcus neoformans protein farnesyltransferase reveal strategies for developing inhibitors that target fungal pathogens. Journal of Biological Chemistry 286: 35149-35162, 2011, PMID 21816822. PMCID: PMC3186413

J Stout, LB Gadkowski, S Rath, JA Alspaugh, MB Miller, GM Cox. Pedicure-associated rapidly growing mycobacterial infection: An endemic disease. Clinical Infectious Diseases 53:787-792, 2011, PMID 21921222.

L Okagaki, Wang Y, ER Ballou, TR O’Meara, Y-S Bahn, JA Alspaugh, C Xue, KS Nielsen. Cryptococcal titan cell formation is regulated by G-protein signaling in response to multiple stimuli. Eukaryotic Cell 10: 1306-1316, 2011, PMCID: PMC3187071.

 K Selvig and JA Alspaugh. pH response pathways in fungi: adapting to host-derived and environmental signals. Mycobiology 39: 249-256, 2011 (Open Acess http://dx.doi.org/10.5941/MYCO.2011.39.4.249). PMCID: PMC3385132

ER Ballou, JA Alspaugh, CB Nichols. “Morphogenesis in Cryptococcus neoformans”. In J Perez-Martin and A Di Pietro, ed. Fungal Morphogenesis. Berlin: Springer-Verlag, 2012.

G Shen, E Zhou, JA Alspaugh, P Wang. Wsp1 is downstream of Cin1 and regulates vesicle transport and actin cytoskeleton as an effector of Cdc42 and Rac1 in Cryptococcus neoformans. Eukaryotic Cell 11:471-481, 2012. PMCID: PMC3318296

TR O’Meara and JA Alspaugh. Cryptococcus neoformans capsule: a sword and a shield. Clinical Microbiology Reviews 12: 387-408, 2012. PMCID: PMC3416491

TR O'Meara, SM Holmer, K Selvig, F Dietrich, and JA Alspaugh. Cryptococcus neoformans Rim101 is associated with cell wall remodeling and evasion of the host immune responses. mBio 4(1) e00522-12. doi:10.1128/mBio.00522.12, 2013. PMCID: PMC3551547.

ER Ballou, K Selvig, JL Narloch, CB Nichols, and JA Alspaugh. Two Rac paralogs regulate polarized growth in the human fungal pathogen Cryptococcus neoformans. Fungal Genetics and Biology, 2013, in press.

ER Ballou, L Kozubowski, CB Nichols, and JA Alspaugh. Ras1 acts through duplicated Cdc42 and Rac proteins to regulate morphogenesis and pathogenesis in the human fungal pathogen Cryptococcus neoformans. PLoS Genetics, 2013, in press