Duke University Medical Center
DUKE BACTERIOLOGY RESEARCH UNIT
Faculty and Research

Raphael Valdivia, PhD
Assistant Professor
Department of Molecular Genetics and Microbiology
biography  •  lab members  •  website

Selected Publications

  • Cocchiaro, J.L., Kumar, Y., Fischer, E.R., Hackstadt, T., and Valdivia, R.H. (2008) Cytoplasmic lipid droplets are translocated into the lumen of the Chlamydia trachomatis parasitophorous vacuole. Proc Natl Acad Sci USA. [Epub ahead of print.]

  • Jorgensen, I. and Valdivia, R.H. (2008) The Pmp-like proteins Pls1 and Pls2 are secreted into the lumen of the Chlamydia trachomatis inclusion. Infect Immun. [Epub ahead of print.]

  • Valdivia, R.H. (2008) Chlamydia effector proteins and new insights into chlamydial cellular microbiology. Curr Opin Microbiol. 11:53-59.

  • Bumann, D. and Valdivia, R.H. (2007) Identification of host-induced pathogen genes by differential fluorescence induction reporter systems. Nat Protoc. 2:770-777.
  • Valdivia, R.H. and Heitman, J. (2007) Endosymbiosis: The evil within. Curr Biol. 17:R408-R410. [Full Text]
  • Kumar, Y., Cochiaro, J., and Valdivia, R.H. (2006) The obligate intracellular pathogen Chlamydia trachomatis targets host lipid droplets. Curr Biol. 16:1646-1651.

  • Sisko, J.L., Spaeth, K., Kumar, Y, and Valdivia, R.H. (2006) Multifunctional analysis of Chlamydia-specific genes in a yeast expression. Mol Microbiol. 60:51-66.
  • Valdivia, R.H., Cormack, B., and Falkow, S. (2006) The uses of green fluorescent protein in prokaryotes. Methods Biochem Anal. 47:163-78.

  • Valdivia, R.H. (2004) Modeling the function of bacterial virulence factors in Saccharomyces cerevisiae. Eukaryotic Cell 3:827-834.

  • Valdivia, R.H. and Schekman, R. (2003) The yeast Rho1p/Pkc1p pathway regulates the transport of chitin synthase III (Chs3p) from internal stores to the plasma membrane. Proc Natl Acad Sci USA 100:10287-10292.
  • Valdivia, R.H., Baggott, D., Chuang, J.C. and Schekman, R. (2002) The yeast clathrin adaptor complex-1 is required for the efficient retention of a subset of late-Golgi membrane proteins. Developmental Cell 2:283-294.

  • MacDaniel T. K. and Valdivia, R.H. (2000) Promising New Tools for Virulence Gene Discovery. In P. Cossart, P. Bouquet, S. Normark and R. Rapouli (ed.) Cellular Microbiology ASM Press, Washington DC p333-345.
  • Valdivia, R.H. and Ramakrishnan, L. (2000) Applications of GFP gene fusions and flow cytometry to the study of bacterial gene expression in host cells. Methods in Enzymology 326:47-73.
  • Cirillo, D.M., Valdivia, R. H., Monack, D. and Falkow, S. (1998) Macrophage-dependent induction of the Salmonella Pathogenicity-Island-2 type III secretion system and its role in intracellular survival. Molecular Microbiology 30:175-188.

  • Valdivia, R. H. and Falkow, S. (1998) Flow cytometry and bacterial pathogenesis. Curr Opin Microbiology 1:359-363.

  • Valdivia, R. H. and Falkow, S. (1997) Fluorescence-based isolation of bacterial genes expressed within host cells. Science 277:2007-2011.