Faculty and Research

Raphael Valdivia, PhD
Associate Professor

Vice Dean for Basic Science
research • biography • publications • lab memberslab website

Representative Publications:

  • Saka HA, Valdivia RH. Emerging Roles for Lipid Droplets in Immunity and Host-Pathogen Interactions. Annu Rev Cell Dev Biol. 2012 May 11. [Epub ahead of print]

  • Valdivia RH. (2012) Thinking outside the box: new strategies for antichlamydial control. Future Microbiol. 7:427-429.

  • Nguyen BD, Valdivia RH. (2012) Virulence determinants in the obligate intracellular pathogen Chlamydia trachomatis revealed by forward genetic approaches. Proc Natl Acad Sci U S A. 109:1263-1268.

  • Saka HA, Thompson JW, Chen YS, Kumar Y, Dubois LG, Moseley MA, Valdivia RH. (2011) Quantitative proteomics reveals metabolic and pathogenic properties of Chlamydia trachomatis developmental forms. Mol Microbiol. 82:1185-1203.

  • Nguyen, B.D., Cunningham, D., Liang, X., Chen, X., Toone, E.J., Raetz, C.R., Zhou, P., and Valdivia, R.H. (2011) Lipooligosaccharide is required for the generation of infectious elementary bodies in Chlamydia trachomatis. Proc Natl Acad Sci U S A. 108:10284-10289.

  • Jorgensen, I., Bednar, M.M., Amin, V., Davis, B.K., Ting, J.P., McCafferty, D.G., and Valdivia, R.H. (2011) The Chlamydia protease CPAF regulates host and bacterial proteins to maintain pathogen vacuole integrity and promote virulence. Cell Host Microbe. 10:21-31.

  • Bednar MM, Jorgensen I, Valdivia RH, McCafferty DG. (2011) Chlamydia protease-like activity factor (CPAF): Characterization of proteolysis activity in vitro and development of a nanomolar affinity CPAF zymogen-serived inhibitor. Biochemistry. 50:7441-7443.

  • Dunn, J.D. and Valdivia, R.H. (2010) Uncivil engineers: Chlamydia, salmonella, and shigella alter cytoskeleton architecture to invade epithelial cells. Future Microbiol. 5:1219-1232.

  • Vignola, M.J., Kashatus, D.F., Taylor, G.A., Counter, C.M., and Valdivia, R.H. (2010) cPLA2 regulates the expression of type I interferons and intracellular immunity to Chlamydia trachomatis. J Biol Chem. 285:21625-21635. [Epub 2010 May 7].

  • Saka, H.A. and Valdivia, R.H. (2010) Acquisition of nutrients by Chlamydiae: Unique challenges of living in an intracellular compartment. Curr Opin Microbiol. 13:4-10. [Epub 2009 Dec 16].
  • Spaeth, K.E., Chen, Y.S. and Valdivia, R.H. (2009) The Chlamydia type III secretion system C-ring engages a chaperone-effector protein complex. PLoS Pathog. 5(9):e1000579. [Epub 2009 Sep 11].

  • Kumar, Y., and Valdivia, R.H. (2009) Leading a sheltered life: Intracellular pathogens and maintenance of vacuolar compartments. Cell Host Microbe. 5:593-601.

  • Kumar, Y., and Valdivia, R.H. (2008) Actin and intermediate filaments stabilize the Chlamydia trachomatis vacuole by forming dynamic structural scaffolds. Cell Host Microbe. 4:159-169.

  • Cocchiaro, J.L., Kumar, Y., Fischer, E.R., Hackstadt, T., and Valdivia, R.H. (2008) Cytoplasmic lipid droplets are translocated into the lumen of Chlamydia trachomatis parasitophorous vacuole. Proc Natl Acad Sci USA. 105:9379-9384.

  • Jorgensen, I. and Valdivia, R.H. (2008) Pmp-like proteins Pls1 and Pls2 are secreted into the lumen of the Chlamydia trachomatis inclusion. Infect Immun. 76:3940-3950. [Epub 2008 Jun 30].
  • Valdivia, R.H. (2008) Chlamydia effector proteins and new insights into chlamydial cellular microbiology. Curr Opin Microbiol. 11:53-59.
  • Bumann, D. and Valdivia, R.H. (2007) Identification of host-induced pathogen genes by differential fluorescence induction reporter systems. Nat Protoc. 2:770-777.

  • Valdivia, R.H. and Heitman, J. (2007) Endosymbiosis: The evil within. Curr Biol. 17:R408-R410. [Full Text]

  • Kumar, Y., Cochiaro, J., and Valdivia, R.H. (2006) The obligate intracellular pathogen Chlamydia trachomatis targets host lipid droplets. Curr Biol. 16:1646-1651.

  • Sisko, J.L., Spaeth, K., Kumar, Y, and Valdivia, R.H. (2006) Multifunctional analysis of Chlamydia-specific genes in a yeast expression. Mol Microbiol. 60:51-66.

  • Valdivia, R.H., Cormack, B., and Falkow, S. (2006) The uses of green fluorescent protein in prokaryotes. Methods Biochem Anal. 47:163-78.

  • Valdivia, R.H. (2004) Modeling the function of bacterial virulence factors in Saccharomyces cerevisiae. Eukaryotic Cell 3:827-834.

  • Valdivia, R.H. and Schekman, R. (2003) The yeast Rho1p/Pkc1p pathway regulates the transport of chitin synthase III (Chs3p) from internal stores to the plasma membrane. Proc Natl Acad Sci USA 100:10287-10292.

  • Valdivia, R.H., Baggott, D., Chuang, J.C. and Schekman, R. (2002) The yeast clathrin adaptor complex-1 is required for the efficient retention of a subset of late-Golgi membrane proteins. Developmental Cell 2:283-294.

  • MacDaniel T. K. and Valdivia, R.H. (2000) Promising New Tools for Virulence Gene Discovery. In P. Cossart, P. Bouquet, S. Normark and R. Rapouli (ed.) Cellular Microbiology ASM Press, Washington DC p333-345.

  • Valdivia, R.H. and Ramakrishnan, L. (2000) Applications of GFP gene fusions and flow cytometry to the study of bacterial gene expression in host cells. Methods in Enzymology 326:47-73.

  • Cirillo, D.M., Valdivia, R. H., Monack, D. and Falkow, S. (1998) Macrophage-dependent induction of the Salmonella Pathogenicity-Island-2 type III secretion system and its role in intracellular survival. Molecular Microbiology 30:175-188.

  • Valdivia, R. H. and Falkow, S. (1998) Flow cytometry and bacterial pathogenesis. Curr Opin Microbiology 1:359-363.

  • Valdivia, R. H. and Falkow, S. (1997) Fluorescence-based isolation of bacterial genes expressed within host cells. Science 277:2007-2011.