Faculty and Research

Mariano Garcia-Blanco, MD, PhD
Charles D. Watts Professor

researchbiographylab members • publications • lab website

Representative Publications:

  • Wagner, E.J. and Garcia-Blanco, M.A. (2001) Polypyrimidine tract binding protein antagonizes exon definition. Mol. Cell. Biol., 21:3281-3288.

  • Wagner, E.J. and Garcia-Blanco, M.A. (2002) RNAi-mediated PTB depletion leads to enhanced exon definition. Molecular Cell, 10:943-949.

  • Colón-Ramos, D. Salisbury, J.L., Sanders, M.A., Shenoy, S.M., Singer, R.H., and García-Blanco, M.A. (2003) Asymmetric distribution of nuclear pore complexes and the cytoplasmic localization of b 2-tubulin mRNA in Chlamydomonas reinhardtii. Developmental Cell, 4:941-952.

  • Garcia-Blanco, M.A. (2003) Mending the message. Nature Biotech., 21:1448-1449.

  • Wollerton, M., Gooding, C., Wagner, E., Garcia-Blanco, M.A., and Smith, C. (2004) Autoregulation of the polypyrimidine tract binding protein by alternative splicing leading to nonsense mediated decay. Molecular Cell, 13: 91-100.

  • Garcia-Blanco, M.A., Baraniak A. and Lasda E. (2004) Alternative splicing in disease and therapy. Nature Biotech., 22:535-546.

  • Florez, P., Sessions, O., Wagner, E., Gromeier, M., and Garcia-Blanco, M.A. (2005) The Polypyrimidine tract binding protein is required for efficient picornavirus gene expression and propagation. J. Virol., 79:6172-6179.

  • Baraniak, A.P., Chen, J.R. and Garcia-Blanco, M.A. (2006) Fox-2 mediates epithelial-specific fibroblast growth factor receptor 2 exon choice. Molecular and Cellular Biology, 26:1209-1222.

  • Oltean, S., Sorg, B.S., Albrecht, T., Bonano, V.I., Brazas, R.M., Dewhirst, M.W., and Garcia-Blanco, M.A. (2006) Alternative inclusion of FGFR2 exon IIIc in Dunning prostate tumors reveals unexpected epithelial mesenchymal plasticity. Proc. Natl, Acad. Sci., 103:14116-14121.

  • Bonano, V.I., Oltean, S., Brazas, R.M. and Garcia-Blanco, M.A. (2006) Imaging the alternative silencing of FGFR2 exon IIIb in vivo. RNA, 12:2073-2079.

  • Gregory, S.G., Schmidt, S., Seth, P., Oksenberg, J.R., Hart, J., Prokop, A., Caillier, S.J., Ban, M., Goris, A., Barcellos, L.F., Lincoln, R., McCauley, J.L., Sawcer, S.J., Compston, A., Dubois, B., Hauser, S.L., Garcia-Blanco, M.A., Pericak-Vance, M.A. and Haines, J.L., for the Multiple Sclerosis Genetics Group. (2007) Interleukin 7 receptor chain (IL7R) shows allelic and functional association with multiple sclerosis. Nature Genetics, 39: 1083-1091. (PMID17660817)

  • Bonano, V., Oltean, S. and Garcia-Blanco, M.A. (2007) A protocol for imaging alternative splicing regulation in vivo using fluorescence reporters in transgenic mice. Nature Protocols, 2(9):2166-81. (PMID17853873)

  • Sessions, O.M., Barrows, N.J., Souza-Neto, J.A., Robinson, T.J., Hersehy, C.L., Rodgers, M.A., Ramirez, J.L., Dimopoulos, G., Yang, P.L., Pearson, J.L., and Garcia-Blanco, M.A. (2009) Discovery of insect and human dengue virus host factors. Nature, 458(7241):1047-1050. (PMID19396146)

  • Anwar, A., Leong, K.M., Ng, M.L., Chu, J.H., and Garcia-Blanco, M.A. (2009) The polypyrimidine tract binding protein is required for efficient dengue virus propagation and associates with the viral repolication machinery. J. Biol. Chem., [Epub ahead of print 2009 Apr 20] (PMID19380576)

  • Marengo, M. and Garcia-Blanco, M.A. (2009) Shedding (UV) light on alternative splicing.  Cell, 137(4):600-2.

  • Barrows, N.J., Sommer, C.L., Garcia-Blanco, M.A., and Pearson J.L. (2010) Factors affecting reproducibility between genome-scale siRNA-based screens. J Biomol Screen. Aug;15(7):735-47. [Epub 2010 Jul 12] (PMID: 20625183)

  • Evsyukova, I., Somarelli, J.A., Gregory, S.G., and Garcia-Blanco M.A. (2010) Alternative splicing in multiple sclerosis and other autoimmune diseases. RNA Biol. Jul 7; 7(4):462-73. [Epub 2010 Jul 1]. (PMCID: PMC3070910)

  • Zhang, X., Nicholls, P.J., Laje, G., Sotnikova, T.D., Gainetdinov, R.R., Albert, P.R., Rajkowska, G., Stockmeier, C.A., Speer, M.C., Steffens, D.C., Austin M.C., McMahon, F.J., Krishnan, K.R., Garcia-Blanco, M.A., and Caron, M.G. (2010) A functional alternative splicing mutation in human tryptophan hydroxylase-2. Mol Psychiatry. Sep 21. [Epub ahead of print] (PMCID: PMC3021090)

  • Miller, H.B., Robinson, T., Gordân, R., Hartemink, A.J., and Garcia-Blanco, M.A. (2011) Identification of Tat-SF1 cellular targets by exon array analysis reveals dual roles in transcription and splicing. RNA, 17(4):665-74. [Epub 2011 Jan 31]. (PMCID: PMC3062177)

  • Armstrong, A., Freedland, S. and Garcia-Blanco, M.A. (2011) Epithelial–mesenchymal transition in prostate cancer: providing new targets for therapy. Asian Journal of Andrology, 13(2):179-80. [Epub 2011 Jan 3]. No abstract available. (PMCID: PMC3157566)

  • Armstrong, A.J., Marengo, M.S., Oltean, S., Kemeny, G., Bitting, R.L., Turnbull, J.D., Herold, C.I., Marcom, P.K., George, D.J., Garcia-Blanco, M.A. (2011) Circulating tumor cells from patients with advanced prostate and breast cancer display both epithelial and mesenchymal markers. Mol Cancer Res. Aug;9(8):997-1007. [Epub 2011 Jun 10]. (PMCID: PMC3157566)

  • Anwar, A., Hosoya, T., Leong, K.M., Onogi, H., Okuno, Y., Hiramatsu, T., Koyama, H., Suzuki, M., Hagiwara, M., Garcia-Blanco, M.A. (2011) The Kinase Inhibitor SFV785 Dislocates Dengue Virus Envelope Protein from the Replication Complex and Blocks Virus Assembly. PLoS One. 2011;6(8):e23246. [Epub 2011 Aug 17]. (PMCID: PMC3157368)

  • Ward, A.M., Bidet, K.,Yinglin, A., Ler, S.G., Hogue, K., Blackstock, W., Gunaratne, J., Garcia-Blanco, M.A. (2011) Quantitative mass spectrometry of DENV-2 RNA-interacting proteins reveals that the DEAD-box RNA helicase DDX6 binds the DB1 and DB2 3’ UTR structures. RNA Biol. 2011 Nov 1;8(6).

  • Robinson, T.J., Forte, E., Salinas, R.E., Puri, S., Marengo, M., Garcia-Blanco, M.A., Luftig M.A. (2012) SplicerEX: a tool for the automated detection and classification of mRNA changes from conventional and splice-sensitive microarray expression data. RNA. 2012 Aug;18(8):1435-45. [Epub 2012 Jun 26].

  • Le Sommer C, Barrows NJ, Bradrick SS, Pearson JL, Garcia-Blanco MA. (2012) G protein-coupled receptor kinase 2 promotes flaviviridae entry and replication.  PLoS Negl Trop Dis. 2012 Sep;6(9):e1820. [Epub 2012 Sep 13]. (PMID: 23029581 PMCID: PMC3441407)

  • Evsyukova, I., Bradrick, S.S., Gregory, S.G., and Garcia-Blanco, M.A. (2013) Cleavage and polyadenylation specificity factor 1 (CPSF1) regulates alternative splicing of interleukin 7 receptor (IL7R) exon 6. RNA, 19(1):103-15. doi: 10.1261/rna.035410.112. [Epub 2012 Nov 14]. (PMID:23151878 PMCID: PMC3527722)

  • Somarelli, J.A., Schaeffer, D., Bosma, R., Bonano V., Sohn, J.W., Kemeny, G., Ettyreddy, A., and Garcia-Blanco, M.A. (2013) Fluorescence-based alternative splicing reporters for the study of epithelial plasticity in vivo. RNA, 19(1):116-27. doi: 10.1261/rna.035097.112. [Epub 2012 Nov 26]. (PMID:23185039 PMCID: PMC3527723)

  • Homa NJ, Salinas R, Forte E, Robinson TJ, Garcia-Blanco MA, Luftig MA. (2013) Epstein-Barr virus induces global changes in cellular mRNA isoform usage that are important for the maintenance of latency. J Virol. 2013 Nov;87(22):12291-301. (PMID: 24027308)

  • Barrows, N., Bradrick, S., Bidet, K., Anwar, A., Ward, A., Jamison, S.F., Levitt, B., LeSommer, C., and Garcia-Blanco, M.A. (2013) Chapter 22. The Interface between Dengue Virus and the Human Host.  In Dengue and Dengue Hemorrhagic Fever, Gubler, Farrar, Ooi, and Vasudevan, eds. CABI, Oxfordshire, UK. (in press)