Duke University Medical Center
research The Garcia-Blanco Lab
  LAB MEMBERS

Mariano Garcia-Blanco
Professor of Molecular Genetics and Microbiology
Professor of Medicine
Director of the Duke Center for RNA Biology
Duke University Medical Center
Durham, N.C., USA

Professor of Emerging Infectious Diseases
Duke-NUS Graduate Medical School
Singapore

Adjunct Professor of Microbiology
Escuela de Medicina
Recinto de Ciencias Medicas
Universidad de Puerto Rico


Mariano Garcia-Blanco 1977 - Harvard College, Cambridge, MA
A.B. in Biochemical Sciences

1984 - Yale University, New Haven, CT
M.D. - Medicine

1988 - Yale University, New Haven, CT
Ph.D. in Molecular Biophysics and Biochemistry

1987-1990 - MIT, Cambridge, MA
Fellow (Sponsor: Phillip A. Sharp)

  Address: Box 3053 (424 CARL)
Duke University Medical Center
Research Drive
Durham, North Carolina 27710

Phone: 919.613.8636
Fax: 919.613.8646
Email: garci001@mc.duke.edu

Biography Mariano A. Garcia-Blanco MD, PhD, is the Charles D. Watts Professor of Molecular Genetics and Microbiology, and Medicine, and Director of the Center for RNA Biology at Duke University in the USA. He is also Professor of Emerging Infectious Diseases at the Duke-NUS Graduate Medical School in Singapore. Professor Garcia-Blanco obtained his AB in Biochemical Sciences at Harvard College, and his MD and PhD (in Molecular Biophysics and Biochemistry) at Yale University. He obtained his postdoctoral training in RNA biology with Nobel laureate Phillip A. Sharp at MIT. Professor Garcia-Blanco is an internationally recognized expert in RNA biology and is an author of more than 120 scientific publications. Additionally, he co-founded Intronn Inc. (now VIRxSYS, Rockville, MD, USA) to develop a novel trans-splicing RNA therapy, and Veri-Q Inc. (part of Proteome Sciences plc, Cobham, UK), to commercialize reagents for the quality control of chemically synthesized nucleic acids, and more recently Singapore Advanced Biologics Pte Ltd (SABio). Professor Garcia-Blanco has served as a member of the Scientific Advisory Board for European Alternative Splicing Network of Excellence (EU), National Advisory General Medical Sciences Council (NIH, USA), and of the Puerto Rico Trust for Science, Research and Technology. He is currently a member of the Council of Scientific Advisers for the International Centre for Genetic Engineering and Biotechnology (United Nations). In 2011 he was elected to the Association of American Physicians (AAP), in 2012 fellow of the American Association for the Advancement of Science, and in 2013 fellow of the American Academy of Microbiology. 

Representative Publications:

  • Wagner, E.J. and Garcia-Blanco, M.A. (2001) Polypyrimidine tract binding protein antagonizes exon definition. Mol. Cell. Biol., 21:3281-3288.

  • Wagner, E.J. and Garcia-Blanco, M.A. (2002) RNAi-mediated PTB depletion leads to enhanced exon definition. Molecular Cell, 10:943-949.

  • Colón-Ramos, D. Salisbury, J.L., Sanders, M.A., Shenoy, S.M., Singer, R.H., and García-Blanco, M.A. (2003) Asymmetric distribution of nuclear pore complexes and the cytoplasmic localization of b 2-tubulin mRNA in Chlamydomonas reinhardtii. Developmental Cell, 4:941-952.

  • Garcia-Blanco, M.A. (2003) Mending the message. Nature Biotech., 21:1448-1449.

  • Wollerton, M., Gooding, C., Wagner, E., Garcia-Blanco, M.A., and Smith, C. (2004) Autoregulation of the polypyrimidine tract binding protein by alternative splicing leading to nonsense mediated decay. Molecular Cell, 13: 91-100.

  • Garcia-Blanco, M.A., Baraniak A. and Lasda E. (2004) Alternative splicing in disease and therapy. Nature Biotech., 22:535-546.

  • Florez, P., Sessions, O., Wagner, E., Gromeier, M., and Garcia-Blanco, M.A. (2005) The Polypyrimidine tract binding protein is required for efficient picornavirus gene expression and propagation. J. Virol., 79:6172-6179.

  • Baraniak, A.P., Chen, J.R. and Garcia-Blanco, M.A. (2006) Fox-2 mediates epithelial-specific fibroblast growth factor receptor 2 exon choice. Molecular and Cellular Biology, 26:1209-1222.

  • Oltean, S., Sorg, B.S., Albrecht, T., Bonano, V.I., Brazas, R.M., Dewhirst, M.W., and Garcia-Blanco, M.A. (2006) Alternative inclusion of FGFR2 exon IIIc in Dunning prostate tumors reveals unexpected epithelial mesenchymal plasticity. Proc. Natl, Acad. Sci., 103:14116-14121.

  • Bonano, V.I., Oltean, S., Brazas, R.M. and Garcia-Blanco, M.A. (2006) Imaging the alternative silencing of FGFR2 exon IIIb in vivo. RNA, 12:2073-2079.

  • Gregory, S.G., Schmidt, S., Seth, P., Oksenberg, J.R., Hart, J., Prokop, A., Caillier, S.J., Ban, M., Goris, A., Barcellos, L.F., Lincoln, R., McCauley, J.L., Sawcer, S.J., Compston, A., Dubois, B., Hauser, S.L., Garcia-Blanco, M.A., Pericak-Vance, M.A. and Haines, J.L., for the Multiple Sclerosis Genetics Group. (2007) Interleukin 7 receptor chain (IL7R) shows allelic and functional association with multiple sclerosis. Nature Genetics, 39: 1083-1091. (PMID17660817)

  • Bonano, V., Oltean, S. and Garcia-Blanco, M.A. (2007) A protocol for imaging alternative splicing regulation in vivo using fluorescence reporters in transgenic mice. Nature Protocols, 2(9):2166-81. (PMID17853873)

  • Sessions, O.M., Barrows, N.J., Souza-Neto, J.A., Robinson, T.J., Hersehy, C.L., Rodgers, M.A., Ramirez, J.L., Dimopoulos, G., Yang, P.L., Pearson, J.L., and Garcia-Blanco, M.A. (2009) Discovery of insect and human dengue virus host factors. Nature, 458(7241):1047-1050. (PMID19396146)

  • Anwar, A., Leong, K.M., Ng, M.L., Chu, J.H., and Garcia-Blanco, M.A. (2009) The polypyrimidine tract binding protein is required for efficient dengue virus propagation and associates with the viral repolication machinery. J. Biol. Chem., [Epub ahead of print 2009 Apr 20] (PMID19380576)

  • Marengo, M. and Garcia-Blanco, M.A. (2009) Shedding (UV) light on alternative splicing.  Cell, 137(4):600-2.

  • Barrows, N.J., Sommer, C.L., Garcia-Blanco, M.A., and Pearson J.L. (2010) Factors affecting reproducibility between genome-scale siRNA-based screens. J Biomol Screen. Aug;15(7):735-47. [Epub 2010 Jul 12] (PMID: 20625183)

  • Evsyukova, I., Somarelli, J.A., Gregory, S.G., and Garcia-Blanco M.A. (2010) Alternative splicing in multiple sclerosis and other autoimmune diseases. RNA Biol. Jul 7; 7(4):462-73. [Epub 2010 Jul 1]. (PMCID: PMC3070910)

  • Zhang, X., Nicholls, P.J., Laje, G., Sotnikova, T.D., Gainetdinov, R.R., Albert, P.R., Rajkowska, G., Stockmeier, C.A., Speer, M.C., Steffens, D.C., Austin M.C., McMahon, F.J., Krishnan, K.R., Garcia-Blanco, M.A., and Caron, M.G. (2010) A functional alternative splicing mutation in human tryptophan hydroxylase-2. Mol Psychiatry. Sep 21. [Epub ahead of print] (PMCID: PMC3021090)

  • Miller, H.B., Robinson, T., Gordân, R., Hartemink, A.J., and Garcia-Blanco, M.A. (2011) Identification of Tat-SF1 cellular targets by exon array analysis reveals dual roles in transcription and splicing. RNA, 17(4):665-74. [Epub 2011 Jan 31]. (PMCID: PMC3062177)

  • Armstrong, A., Freedland, S. and Garcia-Blanco, M.A. (2011) Epithelial–mesenchymal transition in prostate cancer: providing new targets for therapy. Asian Journal of Andrology, 13(2):179-80. [Epub 2011 Jan 3]. No abstract available. (PMCID: PMC3157566)

  • Armstrong, A.J., Marengo, M.S., Oltean, S., Kemeny, G., Bitting, R.L., Turnbull, J.D., Herold, C.I., Marcom, P.K., George, D.J., Garcia-Blanco, M.A. (2011) Circulating tumor cells from patients with advanced prostate and breast cancer display both epithelial and mesenchymal markers. Mol Cancer Res. Aug;9(8):997-1007. [Epub 2011 Jun 10]. (PMCID: PMC3157566)

  • Anwar, A., Hosoya, T., Leong, K.M., Onogi, H., Okuno, Y., Hiramatsu, T., Koyama, H., Suzuki, M., Hagiwara, M., Garcia-Blanco, M.A. (2011) The Kinase Inhibitor SFV785 Dislocates Dengue Virus Envelope Protein from the Replication Complex and Blocks Virus Assembly. PLoS One. 2011;6(8):e23246. [Epub 2011 Aug 17]. (PMCID: PMC3157368)

  • Ward, A.M., Bidet, K.,Yinglin, A., Ler, S.G., Hogue, K., Blackstock, W., Gunaratne, J., Garcia-Blanco, M.A. (2011) Quantitative mass spectrometry of DENV-2 RNA-interacting proteins reveals that the DEAD-box RNA helicase DDX6 binds the DB1 and DB2 3’ UTR structures. RNA Biol. 2011 Nov 1;8(6).

  • Robinson, T.J., Forte, E., Salinas, R.E., Puri, S., Marengo, M., Garcia-Blanco, M.A., Luftig M.A. (2012) SplicerEX: a tool for the automated detection and classification of mRNA changes from conventional and splice-sensitive microarray expression data. RNA. 2012 Aug;18(8):1435-45. [Epub 2012 Jun 26].

  • Le Sommer C, Barrows NJ, Bradrick SS, Pearson JL, Garcia-Blanco MA. (2012) G protein-coupled receptor kinase 2 promotes flaviviridae entry and replication.  PLoS Negl Trop Dis. 2012 Sep;6(9):e1820. [Epub 2012 Sep 13]. (PMID: 23029581 PMCID: PMC3441407)

  • Evsyukova, I., Bradrick, S.S., Gregory, S.G., and Garcia-Blanco, M.A. (2013) Cleavage and polyadenylation specificity factor 1 (CPSF1) regulates alternative splicing of interleukin 7 receptor (IL7R) exon 6. RNA, 19(1):103-15. doi: 10.1261/rna.035410.112. [Epub 2012 Nov 14]. (PMID:23151878 PMCID: PMC3527722)

  • Somarelli, J.A., Schaeffer, D., Bosma, R., Bonano V., Sohn, J.W., Kemeny, G., Ettyreddy, A., and Garcia-Blanco, M.A. (2013) Fluorescence-based alternative splicing reporters for the study of epithelial plasticity in vivo. RNA, 19(1):116-27. doi: 10.1261/rna.035097.112. [Epub 2012 Nov 26]. (PMID:23185039 PMCID: PMC3527723)

  • Homa NJ, Salinas R, Forte E, Robinson TJ, Garcia-Blanco MA, Luftig MA. (2013) Epstein-Barr virus induces global changes in cellular mRNA isoform usage that are important for the maintenance of latency. J Virol. 2013 Nov;87(22):12291-301. (PMID: 24027308)

  • Barrows, N., Bradrick, S., Bidet, K., Anwar, A., Ward, A., Jamison, S.F., Levitt, B., LeSommer, C., and Garcia-Blanco, M.A. (2013) Chapter 22. The Interface between Dengue Virus and the Human Host.  In Dengue and Dengue Hemorrhagic Fever, Gubler, Farrar, Ooi, and Vasudevan, eds. CABI, Oxfordshire, UK. (in press)

     

     

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