Duke University Medical Center
research The Garcia-Blanco Lab
  LAB MEMBERS

Shelton Bradrick, PhD
Assistant Research Professor

Shelton Bradrick

1996 - University of Nebraska Omaha
B.S., Biotechnology

2002 - University of Nebraska Medical Center
Ph.D., Pathology and Microbiology

  Phone: 919.452.4704
Email: shelton.bradrick@duke.edu

Research Interests

My broad research goal is to understand molecular host-pathogen interactions in the context of infection with hepatitis C virus (HCV) and related positive-strand RNA viruses. HCV is a pathogenic human virus that chronically replicates within the liver of infected persons, often resulting in life-threatening complications. The HCV RNA genome has evolved a unique mechanism by which it promotes synthesis of viral proteins. Translation of HCV RNA depends upon an element within the viral 5' untranslated region (UTR) known as the internal ribosome entry site (IRES). The HCV IRES has evolved to efficiently recruit host ribosomes in a 7-methylguanosine cap-independent manner. I am interested in understanding the role of RNA-protein interactions in regulation of HCV IRES activity and virus replication. In addition, I am investigating the biology of interferon-lambdas and their control of HCV infection. My long-term goal is to identify novel host pathways that are manipulated by HCV to benefit viral replication and persistence.

I joined the Garcia-Blanco laboratory as an independent investigator in July of 2009 to continue pursuing lines of research involving molecular interactions between Flaviviridae and the human host. I am currently supported by a Mentored Research Scientist Development Award (K01) from the NIDDK.

Representative Publications

  • S.S. Bradrick*, S. Nagyal and H. Novatt (2013). A miRNA-responsive cell-free translation system facilitates isolation of hepatitis C virus miRNP complexes. RNA, Epub ahead of print. *corresponding author.

  • I. Evsyukova, S.S. Bradrick, S.G. Gregory and M.A. Garcia-Blanco (2013). Cleavage and polyadenylation specificity factor 1 (CPSF1) regulates alternative splicing of interleukin 7 receptor (IL7R) exon 6. RNA, 19(1):103-115.

  • C. Le Sommer, N.J. Barrows, S.S. Bradrick, J.L. Pearson and M.A. Garcia-Blanco (2012). G Protein-coupled Receptor Kinase 2 promotes flaviridae entry and replication. PLoS Neglected Tropical Diseases, 6(9):e1820.
  • D. Piñeiro, J. Ramajo, S.S. Bradrick, and E. Martínez-Salas (2012). Gemin5 proteolysis reveals a novel motif to identify L protease targets. Nucleic Acids Research, 40(11):4942-4953.

  • S.S. Choi*, S. Bradrick*, G. Qiang*, A.M. Diehl, and R. Jhaveri (2011). Upregulation of Hedgehog Pathway is Associated with Cellular Permissiveness for Hepatitis C Virus Replication. Hepatology, 54(5): 1580-1590. *these authors contributed equally.

  • M. Shveygert, C. Kaiser, S.S. Bradrick, and M. Gromeier (2010). Regulation of eIF4E phosphorylation by MAPK occurs through modulation of Mnk1-eIF4G interaction. Molecular and Cellular Biology, 30(21):5160-5167.

  • T.J. Urban*, A.J. Thompson*, S.S. Bradrick*, J. Fellay, D. Schuppan, K.D. Cronin, L. Hong, A. McKenzie, K. Patel, K.V. Shianna, J.G. McHutchison, D.B. Goldstein, and N. Afdhal (2010). IL28B genotype is associated with differential expression of intrahepatic interferon-stimulated genes in chronic hepatitis C patients. Hepatology, 52(6):1888-1896. *these authors contributed equally.

  • T. Pereira, R. Witek, W. Syn, S. Choi, S.S. Bradrick, G. Karaca, K. Agboola, Y. Jung, A. Omenetti, C. Moylan, L. Yang, M. Fernandez-Zapico, R. Jhaveri, V. Shah, F. Pereira, and A.M. Diehl (2010). Viral Factors Induce Hedgehog Pathway Activation in Humans with Viral Hepatitis, Cirrhosis, and Hepatocellular Carcinoma. Laboratory Investigation, 90(12):1690-1703.

  • R.W. Walters, S.S. Bradrick, and M. Gromeier (2010). Poly(A)-binding protein modulates mRNA susceptibility to cap-dependent miRNA-mediated repression. RNA, 16(1): 239-250.

  • S.S. Bradrick* and M. Gromeier (2009). Identification of gemin5 as a novel 7-methylguanosine cap-binding protein. PLOS One, 4(9):e7030. *corresponding author.

  • C. Kaiser, E.Y. Dobrikova, S.S. Bradrick, M. Shveygert, J.T. Herbert and M. Gromeier (2008). Activation of cap-independent translation by variant eukaryotic initiation factor 4G in vivo. RNA, 14(10):2170-2182.

  • S.S. Bradrick*, E. Dobrikova, C. Kaiser, M. Shveygert, and M. Gromeier (2007). Poly(A)-binding protein is differentially required for translation mediated by viral internal ribosome entry sites. RNA, 13(9):1582-1593 *corresponding author.

  • S.S. Bradrick, R.W. Walters and M. Gromeier (2006). The hepatitis C virus 3’ untranslated region or a poly(A) tract promote efficient translation subsequent to the initiation phase. Nucleic Acids Res., 34(4):1293-1303.

  • E. Dobrikova, P. Flores, S.S. Bradrick and M. Gromeier (2003). Activity of a picornavirus internal ribosomal entry site is determined by sequences in the 3’ nontranslated region. Proc. Natl. Acad. Sci. USA, 100(25):15125-15130.

  • J.J. Dunn, S.S. Bradrick, N.M Chapman, S.M. Tracy and J.R. Romero (2003). The stem loop II within the 5' nontranslated region of clinical coxsackievirus B3 genomes determines cardiovirulence phenotype in a murine model. J. Infect. Dis., 187(10):1552-1561.

  • S.S. Bradrick, E.A Lieben, B.M. Carden and J.R. Romero (2001). A predicted secondary structural domain within the internal ribosome entry site of echovirus 12 mediates a cell-type-specific block to viral replication. J. Virol., 75(14):6472-6481.

  • S.S. Bradrick and J.R. Romero (2001). Poliovirus. In: The Encyclopedia of Life Sciences (Nature Publishing Group, Macmillan Reference Ltd.), www.els.net.

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